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The cancer-related protein SSX2 interacts with the human homologue of a Ras-like GTPase interactor, RAB3IP, and a novel nuclear protein, SSX2IP.
Our Abpromise guarantee covers the use of ab10256 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. PubMed: 23826132|
|ELISA||1/16000. Antibody detection limit in Peptide ELISA|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 71 kDa (predicted molecular weight: 100 kDa).Can be blocked with Human SSX2IP peptide (ab23199). Preliminary experiments gave an approx 100-120kDa band in Human Heart lysate at 1ug/ml. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 71kDda according to NP_054740.|
|Flow Cyt||1/100. ab37373-Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.|
|ICC/IF||Use at an assay dependent concentration.|
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