Overview

  • Product name
  • Description
    Goat polyclonal to SSX2IP
  • Tested applications
    Suitable for: IHC-P, ELISA, WB, ICC, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide: SYTNSHVEKDDLP, corresponding to C terminal amino acids 602-614 of Human SSX2IP.

  • Positive control
    • Human heart lysate.
  • General notes


    The cancer-related protein SSX2 interacts with the human homologue of a Ras-like GTPase interactor, RAB3IP, and a novel nuclear protein, SSX2IP.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris-saline. pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Primary antibody notes
    The cancer-related protein SSX2 interacts with the human homologue of a Ras-like GTPase interactor, RAB3IP, and a novel nuclear protein, SSX2IP.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab10256 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. PubMed: 23826132
ELISA 1/16000. Antibody detection limit in Peptide ELISA
WB Use a concentration of 1 µg/ml. Detects a band of approximately 71 kDa (predicted molecular weight: 100 kDa).Can be blocked with Human SSX2IP peptide (ab23199). Preliminary experiments gave an approx 100-120kDa band in Human Heart lysate at 1ug/ml. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 71kDda according to NP_054740.
ICC 1/100.
Flow Cyt 1/100. ab37373-Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration.

Target

  • Function
    Belongs to an adhesion system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). May connect the nectin-afadin and E-cadherin-catenin system through alpha-actinin and may be involved in organization of the actin cytoskeleton at AJs through afadin and alpha-actinin.
  • Tissue specificity
    Widely expressed, with the highest expression in brain, intermediate expression in kidney, testis, spinal cord, liver, heart, lung, skeletal muscle, ovary, fetal liver and fetal brain, and little to no expression in pancreas and spleen. All specific brain regions showed intermediate to high expression, with highest expression in amygdala. Also expressed in fetal tissues, mainly in liver and brain.
  • Domain
    Both the N-terminal (up to position 79) and the C-terminal (from position 304) sequences are required for interaction with SSX2.
  • Cellular localization
    Cell junction > adherens junction. Nucleus. Not found at cell-matrix AJs.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADIP antibody
    • ADIP_HUMAN antibody
    • Afadin and alpha actinin binding protein antibody
    • Afadin DIL domain interacting protein antibody
    • Afadin DIL domain-interacting protein antibody
    • Afadin- and alpha-actinin-binding protein antibody
    • FLJ10848 antibody
    • KIAA0923 antibody
    • MGC75026 antibody
    • OTTHUMP00000011574 antibody
    • SSX 2 interacting protein antibody
    • SSX2 interacting protein antibody
    • SSX2-interacting protein antibody
    • SSX2IP antibody
    • Synovial sarcoma X breakpoint 2 interacting protein antibody
    see all

Images

  • ab10256 (1/100) detecting SSX2IP in a variety of Human cell lines. Please refer to abreview for further details. This image is part of the following publication: Denniss, F.A.K., Breslin, A., Ingram, W.I., Hardwick, N.H., Mufti, G.J. & Guinn, B.A. (2007) The leukaemia associated antigen, SSX2IP, is expressed during mitosis on the surface of myeloid leukaemia cells. In Press, British Journal of Haematology

    See Abreview

  • ab10256 (1/100) stainning SSX2IP in K562 (myeloid leukaemia cell line) by flow cytometry. Please refer to Abreview for further experimental details. This image is part of the following publication: Denniss, F.A.K., Breslin, A., Ingram, W.I., Hardwick, N.H., Mufti, G.J. & Guinn, B.A. (2007) The leukaemia associated antigen, SSX2IP, is expressed during mitosis on the surface of myeloid leukaemia cells. In Press, British Journal of Haematology

    See Abreview

  • ab10256 (1/100) stainning SSX2IP in human myeloid cells (green). Cells were fixed in paraformaldehyde and counterstained with DAPI (blue) in order to highlight the nucleus. Please refer to abreview for further experimental details. This image is part of the following publication: Denniss, F.A.K., Breslin, A., Ingram, W.I., Hardwick, N.H., Mufti, G.J. & Guinn, B.A. (2007) The leukaemia associated antigen, SSX2IP, is expressed during mitosis on the surface of myeloid leukaemia cells. In Press, British Journal of Haematology

    See Abreview

References

This product has been referenced in:
  • Li P  et al. Epigenetic silencing of miR-338-3p contributes to tumorigenicity in gastric cancer by targeting SSX2IP. PLoS One 8:e66782 (2013). IHC-P ; Human . Read more (PubMed: 23826132) »
  • Fukumoto Y  et al. Role of Scaffold Protein Afadin Dilute Domain-interacting Protein (ADIP) in Platelet-derived Growth Factor-induced Cell Movement by Activating Rac Protein through Vav2 Protein. J Biol Chem 286:43537-48 (2011). ICC/IF, WB ; Mouse . Read more (PubMed: 22027834) »

See all 3 Publications for this product

Customer reviews and Q&As

Thank you for contacting us.

Please check the datasheet and selct on basis of conc, price and volume. The compatible products are ab102345, ab102354, ab5755, ab97100, ab97105 and ab6741.

I hope this information is helpful to you. ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Human (myeloid cells)
Specification
myeloid cells
Fixative
Methanol
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1.5% · Temperature: 25°C
Username

Dr. Barbara Guinn

Verified customer

Submitted Jul 27 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (myeloid leukaemia cell - K562)
Specification
myeloid leukaemia cell - K562
Preparation
Cell harvesting/tissue preparation method: Centrifugation
Sample buffer: RPMI and 10% foetal calf serum (FCS)
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
Single population of live cells
Username

Dr. Barbara Guinn

Verified customer

Submitted Jun 14 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Human Cultured Cells (Myeloid cells)
Specification
Myeloid cells
Fixative
Methanol
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1.5% · Temperature: 25°C
Username

Dr. Barbara Guinn

Verified customer

Submitted Jun 14 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Myeloid cells)
Specification
Myeloid cells
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Dr. Barbara Guinn

Verified customer

Submitted Jun 14 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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