ST14 antibody - Aminoterminal end cleaved protein (ab28265)

Product Name 

ST14 antibody - Aminoterminal end cleaved protein

Product type 

Primary antibodies

Description 

Rabbit polyclonal to ST14 - Aminoterminal end cleaved protein

Immunogen 

This antibody is made to a synthetic peptide based on the amino end of shed human ST14.(Peptide available as ab41265.)

Reacts with 

Hu

Predicted to react 
(species key)

Ms, Rat
(Due to sequence homology)

Specificity 

Ab28265 reacts with ST14, and does not recognize matriptase2.

We have a range of domain specific antibodies for this target. For a full list please see all ST14 antibodies

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: Use at an assay dependent dilution. Predicted molecular weight: 95 kDa. Nonreduced SDS-PAGE Western blots yield bands of 110-95 kDa for ST14 complexed with HAI1 and a 70 kDa band for the single-chain form. The 95 kDa complex resolves to A and B chains of 45 and 25 kDa for ST14, plus a 36 kDa band for the HAI1. The single-chain form is produced by cleavage in the SEA domain, between the stem and the CUB domains and the catalytic domain is liberated by cleavage after the LRLR domains. A recommended starting concentration for Western blots is 1/1,000 when using colorimetric substrates such as BCIP/NBT, and 1/5,000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Membrane; single-pass type II membrane protein

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Matriptase
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Other
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Other Enzymes

Relevance 

ST14 degrades extracellular matrix. It is proposed to play a role in breast cancer invasion and metastasis. ST14 was first identified as a gelatinolytic proteinase in the media and cell lysates of breast tumor cells in culture. Originally it was thought to be specific for either prostate tumors or breast tumors, or to be a tumor suppressor. More extensive characterizations determined that ST14 is found in many normal and tumor cell lines, and in breast milk, serum and urine. ST14 is a Type II serine proteinase, meaning it contains a transmembrane motif in the amino end of the protein. The latent protein is membrane attached, and has a cytoplasmic tail. The extracellular domains contain a stem region, followed by two CUB domains and four repeats of LDL receptor like homology, and a serine protease domain located in the carboxyterminal end of the protein. ST14 is a member of the S1A family of the PA clan of serine proteinases, which includes acrosin, kallikreins, trypsin, chymotrypsin, and many other important enzymes.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Customer reviews (feedback) regarding ST14 antibody - Aminoterminal end cleaved protein

Customer FAQs regarding ST14 antibody - Aminoterminal end cleaved protein

Protocols for ST14 antibody - Aminoterminal end cleaved protein

Price and availability of products related to ST14 antibody - Aminoterminal end cleaved protein

ST14 antibody - Aminoterminal end cleaved protein - related products: