Anti-ST14 antibody - Catalytic domain (ab28266)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab28266 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB Use at an assay dependent dilution. Predicted molecular weight: 95 kDa. A recommended starting concentration for Western blots is 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates.

Target

  • FunctionDegrades extracellular matrix. Proposed to play a role in breast cancer invasion and metastasis. Exhibits trypsin-like activity as defined by cleavage of synthetic substrates with Arg or Lys as the P1 site.
  • Involvement in diseaseDefects in ST14 are a cause of ichthyosis autosomal recessive with hypotrichosis (ARIH) [MIM:610765]. ARIH is a skin disorder characterized by congenital ichthyosis associated with the presence of less than the normal amount of hair.
  • Sequence similaritiesBelongs to the peptidase S1 family.
    Contains 2 CUB domains.
    Contains 4 LDL-receptor class A domains.
    Contains 1 peptidase S1 domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Epithin antibody
    • HAI antibody
    • Matriptase antibody
    • Membrane type serine protease 1 antibody
    • Membrane type serine protease antibody
    • Membrane-type serine protease 1 antibody
    • MT SP1 antibody
    • MT-SP1 antibody
    • MTSP 1 antibody
    • MTSP1 antibody
    • Prostamin antibody
    • PRSS 14 antibody
    • PRSS14 antibody
    • Serine protease 14 antibody
    • Serine protease TADG 15 antibody
    • Serine protease TADG-15 antibody
    • Serine protease TADG15 antibody
    • SNC 19 antibody
    • SNC19 antibody
    • ST 14 antibody
    • ST14 antibody
    • ST14_HUMAN antibody
    • Suppression of tumorigenicity 14 (colon carcinoma matriptase epithin) antibody
    • Suppression of tumorigenicity 14 (colon carcinoma) antibody
    • Suppression of tumorigenicity 14 antibody
    • Suppressor of tumorigenicity 14 protein antibody
    • Suppressor of tumorigenicity protein 14 antibody
    • TADG 15 antibody
    • TADG15 antibody
    • TMPRSS14 antibody
    • Tumor associated differentially expressed gene 15 protein antibody
    • Tumor-associated differentially-expressed gene 15 protein antibody
    see all

Anti-ST14 antibody - Catalytic domain images

  • ICC/IF image of ab28266 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28266, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-ST14 antibody - Catalytic domain (ab28266)

ab28266 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. After having discussion with my lab colleagues, regrettably I have to say we have not tested the cross reactivity of these antibodies with Matriptase 2. We do not have any data for sharing. The matriptase 2 non reac...

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