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Immunocytochemistry/ Immunofluorescence-ST14 antibody - Catalytic domain(ab28266)
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Product Name
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ST14 antibody - Catalytic domain
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See all ST14 antibodies (6)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to ST14 - Catalytic domain
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Immunogen
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Synthetic peptide based on the catalytic domain of Human ST14.
(Peptide available as ab41264.)
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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This antibody does not recognize matriptase 2 (MTSP2).
We have a range of domain specific antibodies for this target. For a full list please see all ST14 antibodies
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 1 µg/ml.
WB: 1/1,000 when using colorimetric substrates such as BCIP/NBT and 1/5,000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. Predicted molecular weight: 95 kDa. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Membrane; single-pass type II membrane protein
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Research areas
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Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Matriptase
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Other
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Other Enzymes
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Relevance
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ST14 degrades extracellular matrix. It is proposed to play a role in breast cancer invasion and metastasis. ST14 was first identified as a gelatinolytic proteinase in the media and cell lysates of breast tumor cells in culture. Originally it was thought to be specific for either prostate tumors or breast tumors, or to be a tumor suppressor. More extensive characterizations determined that ST14 is found in many normal and tumor cell lines, and in breast milk, serum and urine. ST14 is a Type II serine proteinase, meaning it contains a transmembrane motif in the amino end of the protein. The latent protein is membrane attached, and has a cytoplasmic tail. The extracellular domains contain a stem region, followed by two CUB domains and four repeats of LDL receptor like homology, and a serine protease domain located in the carboxyterminal end of the protein. ST14 is a member of the S1A family of the PA clan of serine proteinases, which includes acrosin, kallikreins, trypsin, chymotrypsin, and many other important enzymes.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ST14 antibody - Catalytic domain is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ST14 antibody - Catalytic domain images, references, products related to ab28266 and other tools.
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ST14 antibody - Catalytic domain images:
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 Immunocytochemistry/ Immunofluorescence-ST14 antibody - Catalytic domain(ab28266)
ICC/IF image of ab28266 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28266, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Search PubMed (MEDLINE) for references to ST14
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