ST14 antibody - Cytoplasmic domain (ab28268)

Product Name 

ST14 antibody - Cytoplasmic domain

Product type 

Primary antibodies

Description 

Rabbit polyclonal to ST14 - Cytoplasmic domain

Immunogen 

Synthetic peptide corresponding to the cytoplasmic domain of Human ST14.(Peptide available as ab41262.)

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

ab28268 recognises the Cytoplasmic Domain of ST14.
This antibody does not recognize matriptase 2.

We have a range of domain specific antibodies for this target. For a full list please see all ST14 antibodies

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT - 1/5000 when using chemiluminescent substrates. Predicted molecular weight: 95 kDa. Note: EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Membrane; single-pass type II membrane protein

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Matriptase
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Other
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Other Enzymes

Relevance 

ST14 degrades extracellular matrix. It is proposed to play a role in breast cancer invasion and metastasis. ST14 was first identified as a gelatinolytic proteinase in the media and cell lysates of breast tumor cells in culture. Originally it was thought to be specific for either prostate tumors or breast tumors, or to be a tumor suppressor. More extensive characterizations determined that ST14 is found in many normal and tumor cell lines, and in breast milk, serum and urine. ST14 is a Type II serine proteinase, meaning it contains a transmembrane motif in the amino end of the protein. The latent protein is membrane attached, and has a cytoplasmic tail. The extracellular domains contain a stem region, followed by two CUB domains and four repeats of LDL receptor like homology, and a serine protease domain located in the carboxyterminal end of the protein. ST14 is a member of the S1A family of the PA clan of serine proteinases, which includes acrosin, kallikreins, trypsin, chymotrypsin, and many other important enzymes.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

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