Anti-ST2 antibody (ab233433)
Key features and details
- Rabbit polyclonal to ST2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-ST2 antibody
See all ST2 primary antibodies -
Description
Rabbit polyclonal to ST2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant fragment (His-tag) corresponding to Human ST2 aa 150-350. (Expressed in E.coli).
Database link: Q01638 -
Positive control
- WB: K562, A549 and HEK-293T cell lysates; Human lung lysate; Recombinant human ST2 protein. ICC/IF: A549 cells. IHC-P: Human kidney tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 44.12% PBS, 55.77% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233433 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 63 kDa.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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ICC/IF |
Use a concentration of 5 - 20 µg/ml.
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Notes |
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WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 63 kDa. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
ICC/IF
Use a concentration of 5 - 20 µg/ml. |
Target
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Function
Receptor for interleukin-33 (IL-33), its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Possibly involved in helper T-cell function. -
Tissue specificity
Highly expressed in kidney, lung, placenta, stomach, skeletal muscle, colon and small intestine. Isoform A is prevalently expressed in the lung, testis, placenta, stomach and colon. Isoform B is more abundant in the brain, kidney and the liver. Isoform C is not detected in brain, heart, liver, kidney and skeletal muscle. -
Sequence similarities
Belongs to the interleukin-1 receptor family.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 TIR domain. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 9173 Human
- Omim: 601203 Human
- SwissProt: Q01638 Human
- Unigene: 66 Human
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Alternative names
- DER-4 antibody
- DER4 antibody
- FIT 1 antibody
see all
Images
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Anti-ST2 antibody (ab233433) at 2 µg/ml + Human lung lysate
Predicted band size: 63 kDa -
A549 (human lung carcinoma cell line) cells stained for ST2 using ab233433 at 20 µg/mL in ICC/IF. FITC-labeled secondary antibody.
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Anti-ST2 antibody (ab233433) at 2 µg/ml + HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Predicted band size: 63 kDa -
Anti-ST2 antibody (ab233433) at 2 µg/ml + A549 (human lung carcinoma cell line) cell lysate
Predicted band size: 63 kDa -
Anti-ST2 antibody (ab233433) at 2 µg/ml + K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Predicted band size: 63 kDa -
Formalin-fixed, paraffin-embedded human kidney tissue stained for ST2 using ab233433 at 20 µg/mL in immunohistochemical analysis. DAB staining.
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Anti-ST2 antibody (ab233433) at 2 µg/ml + Recombinant human ST2 protein
Predicted band size: 63 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab233433 has been referenced in 2 publications.
- Huang N et al. IL-33/ST2 promotes the malignant progression of gastric cancer via the MAPK pathway. Mol Med Rep 23:N/A (2021). PubMed: 33760194
- Gao S & Li J Development of a Novel Homogeneous Nanoparticle-Based Assay for Rapid and High-Throughput Quantitation of the sST2 Protein in Human Serum. Int J Nanomedicine 15:10539-10546 (2020). PubMed: 33408473