Recombinant
RabMAb

Anti-ST3GAL3 antibody [EPR10006(2)] (ab177959)

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Overview

  • Product name
    Anti-ST3GAL3 antibody [EPR10006(2)]
  • Description
    Rabbit monoclonal [EPR10006(2)] to ST3GAL3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ST3GAL3 aa 100-200 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q11203

  • Positive control
    • Fetal heart and human skeletal muscle lysates; fetal cardiac muscle tissue; Human skeletal muscle tissue; A673 cells; permealized Jurkat cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR10006(2)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab177959 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
ICC/IF 1/50 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for IP.

    • Target

    • Function
      Catalyzes the formation of the NeuAc-alpha-2,3-Gal-beta-1,4-GlcNAc-, NeuAc-alpha-2,3-Gal-beta-1,3-GlcNAc- or NeuAc-alpha-2,3-Gal-beta-1,3-GalNAc- sequences found in terminal carbohydrate groups of glycoproteins and glycolipids. The highest activity is toward Gal-beta-1,3-GlcNAc and the lowest toward Gal-beta-1,3-GalNAc.
    • Tissue specificity
      Highly expressed in adult skeletal muscle and in all fetal tissues examined and to a much lesser extent in placenta, lung and liver.
    • Pathway
      Protein modification; protein glycosylation.
    • Involvement in disease
      Mental retardation, autosomal recessive 12
      Epileptic encephalopathy, early infantile, 15
    • Sequence similarities
      Belongs to the glycosyltransferase 29 family.
    • Post-translational
      modifications
      The soluble form derives from the membrane form by proteolytic processing.
    • Cellular localization
      Golgi apparatus, Golgi stack membrane. Secreted. Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid.
    • Information by UniProt
    • Database links
      see all
    • Alternative names
      • 3 sialyltransferase antibody
      • 3(4) GlcNAc alpha-2 antibody
      • 3-sialyltransferase 3 antibody
      • 3-sialyltransferase antibody
      • 3-ST 3 antibody
      • 4-galactoside alpha-2 antibody
      • 4GlcNAc alpha 2 3 sialyltransferase antibody
      • Alpha 2 3 sialyltransferase II antibody
      • Alpha 2 3 sialyltransferase III antibody
      • Alpha 2 3 ST 3 antibody
      • Alpha 2 antibody
      • Beta galactoside alpha 3 sialyltransferase 3 antibody
      • Beta-galactoside alpha-2 antibody
      • CMP N acetylneuraminate beta 1 4 galactoside alpha 2 3 sialyltransferase antibody
      • CMP-N-acetylneuraminate-beta-1 antibody
      • EC 2.4.99.6 antibody
      • Gal beta 1 3 antibody
      • Gal beta 1 3(4) GlcNAc alpha 2 3 sialyltransferase antibody
      • Gal beta 1 3(4)GlcNAc alpha 2 3 sialyltransferase antibody
      • Gal beta-1 antibody
      • N acetyllactosaminide alpha 2 3 sialyltransferase antibody
      • N-acetyllactosaminide alpha-2 antibody
      • OTTHUMP00000008820 antibody
      • OTTHUMP00000008821 antibody
      • OTTHUMP00000008822 antibody
      • OTTHUMP00000008823 antibody
      • Sialyltransferase 6 (N acetyllacosaminide alpha 2 3 sialyltransferase) antibody
      • Sialyltransferase 6 antibody
      • SIAT6 antibody
      • SIAT6_HUMAN antibody
      • ST3 beta galactoside alpha 2 3 sialyltransferase 3 antibody
      • ST3 beta galactoside alpha 2,3 sialyltransferase 3 antibody
      • ST3Gal III antibody
      • St3gal3 antibody
      • ST3GALII antibody
      • ST3GalIII antibody
      • ST3N antibody
      see all

    Images

    • Flow Cytometry - Anti-ST3GAL3 [EPR10006(2)] antibody (ab177959)
      Flow Cytometry - Anti-ST3GAL3 [EPR10006(2)] antibody (ab177959)

      Flow cytometric analysis of permeabilized Jurkat cells labeling ST3GAL3 using ab177959 at 1/10 dilution (red), or a rabbit IgG (negative) (green).

    • Immunocytochemistry/ Immunofluorescence - Anti-ST3GAL3 [EPR10006(2)] antibody (ab177959)
      Immunocytochemistry/ Immunofluorescence - Anti-ST3GAL3 [EPR10006(2)] antibody (ab177959)

      Immunofluorescence analysis of A673 cells labeling ST3GAL3 using ab177959 at 1/50 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST3GAL3 [EPR10006(2)] antibody (ab177959)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST3GAL3 [EPR10006(2)] antibody (ab177959)

      Immunohistochemical analysis of Human skeletal muscle tissue labeling ST3GAL3 using ab177959 at 1/250 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST3GAL3 [EPR10006(2)] antibody (ab177959)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST3GAL3 [EPR10006(2)] antibody (ab177959)

      Immunohistochemical analysis of fetal cardiac muscle tissue labeling ST3GAL3 using ab177959 at 1/250 dilution.

    • Western blot - Anti-ST3GAL3 [EPR10006(2)] antibody (ab177959)
      Western blot - Anti-ST3GAL3 [EPR10006(2)] antibody (ab177959)
      All lanes : Anti-ST3GAL3 antibody [EPR10006(2)] (ab177959) at 1/1000 dilution

      Lane 1 : Human fetal heart lysate
      Lane 2 : Human skeletal muscle lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 42 kDa

    References

    ab177959 has not yet been referenced specifically in any publications.

    Publishing research using ab177959? Please let us know so that we can cite the reference in this datasheet.

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