Overview

  • Product nameAnti-ST3GAL4 antibody
    See all ST3GAL4 primary antibodies
  • Description
    Rabbit polyclonal to ST3GAL4
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Chimpanzee
  • Immunogen

    Synthetic peptide corresponding to a region within internal amino acids 252-301 (KQKPTTGLLA ITLALHLCDL VHIAGFGYPD AYNKKQTIHY YEQITLKSMA) of Human ST3GAL4 (NP_006269).

  • Positive control
    • 721_B cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab87114 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 38 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • RelevanceST3GAL4 belongs to the glycosyltransferase 29 family. It may catalyze the formation of the NeuAc-alpha-2,3-Gal-beta-1,3-GalNAc- or NeuAc-alpha-2,3-Gal-beta-1,3-GlcNAc-sequences found in terminal carbohydrate groups of glycoproteins and glycolipids. It may also be involved in the biosynthesis of the sialyl Lewis X determinant. ST3GAL4 is highly expressed in adult placenta, ovary and testes. There are five named isoforms.
  • Cellular localizationGolgi apparatus, Golgi stack membrane; Single-pass type II membrane protein and secreted. Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid.
  • Database links
  • Alternative names
    • Alpha 2 3 sialyltransferase IV antibody
    • Alpha 2 3 ST antibody
    • Alpha 3 N acetylneuraminyltransferase antibody
    • Beta galactoside alpha 2 3 sialyltransferase antibody
    • CGS23 antibody
    • CMP N acetylneuraminate beta galactosamide alpha 2 3 sialyltransferase antibody
    • FLJ11867 antibody
    • FLJ46764 antibody
    • Gal NAc6S antibody
    • NANTA3 antibody
    • SAT 3 antibody
    • SAT3 antibody
    • Sialyltransferase 4C (beta galactosidase alpha 2 3 sialytransferase) antibody
    • Sialyltransferase 4C (beta galactoside alpha 2 3 sialytransferase) antibody
    • SIAT4 antibody
    • SIAT4 C antibody
    • SIAT4C antibody
    • ST 4 antibody
    • ST3 beta galactoside alpha 2 3 sialyltransferase 4 antibody
    • ST3Gal III antibody
    • ST3GalIV antibody
    • ST4 antibody
    • STZ antibody
    see all

Anti-ST3GAL4 antibody images

  • Anti-ST3GAL4 antibody (ab87114) at 1 µg/ml + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 38 kDa
    Observed band size : ~38 kDa (why is the actual band size different from the predicted?)

References for Anti-ST3GAL4 antibody (ab87114)

ab87114 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Lewis Lung Carcinoma cells (LLC1))
Loading amount 10 µg
Specification Lewis Lung Carcinoma cells (LLC1)
Gel Running Conditions Non-reduced Non-Denaturing (Native) (4-20%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
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Submitted Aug 17 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"