1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionCatalyzes the formation of ganglioside GM3 (alpha-N-acetylneuraminyl-2,3-beta-D-galactosyl-1, 4-beta-D-glucosylceramide).
Tissue specificityUbiquitous. High expression in brain, skeletal muscle, placenta, and testis.
Involvement in diseaseDefects in ST3GAL5 are the cause of Amish infantile epilepsy syndrome (AIES) [MIM:609056]. AIES is an autosomal recessive, infantile-onset symptomatic epilepsy associated with developmental stagnation and blindness.
Sequence similaritiesBelongs to the glycosyltransferase 29 family.
Immunohistochemistry analysis in formalin fixed and paraffin embedded Human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. ab107534 at a dilution of 1/50.
References for Anti-ST3GAL5 antibody (ab107534)
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