Overview

  • Product nameAnti-ST4S6 antibody
  • Description
    Rabbit polyclonal to ST4S6
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide: FFYDNSTDGE PPFLTQDFIH AFQPNARLIV MLRDPVERLY SDYLYFASSN , corresponding to internal sequence amino acids 360-409 of Human ST4S6

  • Positive control
    • HeLa cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab80887 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/12500.

Target

  • RelevanceST4S6 is a membrane-integrated glycoprotein that is expressed as a disulfide-linked dimer in B cells. It may act as a B-cell receptor involved in BCR ligation-mediated early activation, which mediates regulatory signals key to B-cell development and/or regulation of B-cell-specific RAG expression, however such results are unclear in vivo.
  • Cellular localizationGolgi apparatus membrane; Single-pass type II membrane protein. Note= A small fraction may also be present at the cell surface, where it acts as a B-cell receptor.
  • Database links
  • Alternative names
    • B cell RAG associated gene protein antibody
    • B cell RAG associated protein (GALNAC4S 6ST) antibody
    • B cell RAG associated protein antibody
    • BRAG antibody
    • GALNAC4S 6ST antibody
    • hBRAG antibody
    • N acetylgalactosamine 4 sulfate 6 O sulfotransferase antibody
    see all

Anti-ST4S6 antibody images

  • Anti-ST4S6 antibody (ab80887) at 1 µg/ml + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 65 kDa
    Observed band size : 65 kDa

References for Anti-ST4S6 antibody (ab80887)

ab80887 has not yet been referenced specifically in any publications.

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