Overview

  • Product nameAnti-ST5 antibody
    See all ST5 primary antibodies
  • Description
    Rabbit polyclonal to ST5
  • Tested applicationsSuitable for: ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to Human ST5 aa 1121-1137 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    KFLRGLGNKMKFLHKKN

  • Positive control
    • Human spleen tissue

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab64897 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1 - 1/1000.
IHC-P Use a concentration of 5 µg/ml.

Target

  • FunctionMay be involved in cytoskeletal organization and tumorgenicity. Isoform 1 seems to be involved in a signaling transduction pathway leading to activation of MAPK1/ERK2. Isoform 3 may block ERK2 activation stimulated by ABL1. Isoform 3 may alter cell morphology and cell growth.
  • Tissue specificityWidely expressed with the exception of peripheral blood lymphocytes. Isoform 1 is expressed in several epithelial and fibroblast (including tumorigenic) but absent in lymphoid cell lines (at protein level). Isoform 3 is expressed in primary cell or weakly tumorigenic but not in tumorigenic cell lines (at protein level).
  • Sequence similaritiesContains 1 dDENN domain.
    Contains 1 DENN domain.
    Contains 1 uDENN domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • DENND2B antibody
    • DENN domain containing protein 2B antibody
    • DENN domain-containing protein 2B antibody
    • DENN/MADD domain containing 2B antibody
    • HeLa tumor suppression 1 antibody
    • HTS1 antibody
    • p126 antibody
    • ST 5 antibody
    • ST5 antibody
    • ST5_HUMAN antibody
    • Suppression of tumorigenicity 5 antibody
    • Suppression of tumorigenicity 5 protein antibody
    see all

Anti-ST5 antibody images

  • This image shows formalin fixed, paraffin embedded human spleen tissue stained with ab64897 at 5µg/ml.

References for Anti-ST5 antibody (ab64897)

This product has been referenced in:
  • O'Bryan MK  et al. RBM5 is a male germ cell splicing factor and is required for spermatid differentiation and male fertility. PLoS Genet 9:e1003628 (2013). WB, IF, IHC ; Mouse . Read more (PubMed: 23935508) »

See 1 Publication for this product

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Thank you for submitting an Abreview of ab64897. As you may have noticed, your review has now been published on our website.

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Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Human Purified protein (ovarian)
Loading amount 50 µg
Specification ovarian
Gel Running Conditions Reduced Denaturing (8%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted Apr 12 2012

Thank you for your interest in the Anti-ST5 antibody ab64897. I apologize for the delay in sending the discount code we discussed.

I am very pleased to hear you would like to test ab64897 in western blotting. This code will give you 1 free p...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"