The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
Use a concentration of 2 - 5 µg/ml.
Use a concentration of 0.01 - 0.1 µg/ml.
RelevanceSialyltransferases are enzymes that transfer sialic acid to nascent oligosaccharide. Each silaytransferase is specific for a particular sugar substrate. Sialytransferases add sialic acid to the terminal portions of the sialylated glycolipids or to the N-or O-linked sugar chases of glycoproteins. The animal sialyltransferases are Golgi type II transmembrane glycosyltransferases. While the enzyme ST6Gal I uses the Gal beta 1-4GlcNAc-R as the acceptor substrate which present in insects.