Anti-ST6GALNAC5 antibody (ab69855)

Overview

  • Product nameAnti-ST6GALNAC5 antibody
    See all ST6GALNAC5 primary antibodies
  • Description
    Rabbit polyclonal to ST6GALNAC5
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cat, Dog, Pig, Chimpanzee, Zebrafish
  • Immunogen

    Synthetic peptide: KGQVYNNLHL LSQVLPRLKA FMITRHKMLQ FDELFKQETG KDRKISNTWL, corresponding to amino acids 182-231 of Human ST6GALNAC5

  • Positive control
    • Jurkat cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab69855 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • RelevanceST6GALNAC5 belongs to a family of sialyltransferases that modify proteins and ceramides on the cell surface to alter cell-cell or cell-extracellular matrix interactions.
  • Cellular localizationGolgi apparatus membrane; Single pass type II membrane protein
  • Database links
  • Alternative names
    • Alpha N acetylgalactosaminide alpha 2,6 sialyltransferase 5 antibody
    • GalNAc alpha 2,6 sialyltransferase V antibody
    • GD1 alpha synthase antibody
    • Sialyltransferase 7E antibody
    • Sialyltransferase E antibody
    • SIAT7 E antibody
    • SIAT7E antibody
    • ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 5 antibody
    • ST6GalNAc V antibody
    see all

Anti-ST6GALNAC5 antibody images

  • Anti-ST6GALNAC5 antibody (ab69855) at 1.25 µg/ml + Jurkat cell lysate at 10 µg with 5% skim milk / PBS buffer

    Secondary
    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size : 39 kDa
    Observed band size : 39 kDa
  • Anti-ST6GALNAC5 antibody (ab69855) at 1/5000 dilution (in PBS + 0.1% Tween + 5% milk powder for 16 hours at 4°C) + Whole cell lysate of Human WT MDA MB231 cells at 20 µg

    Secondary
    An HRP-polymer Goat anti-rabbit polyclonal at 1/10000 dilution
    Developed using the ECL technique

    Performed under non-reducing conditions.

    Predicted band size : 39 kDa
    Observed band size : 39 kDa


    Exposure time : 30 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-ST6GALNAC5 antibody (ab69855)

ab69855 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your email and the additional information and the image.
From the image, it seems that either too much protein, too much primary or too much secondary antibody is being used or the blot is very overexposed, as the bands are too dark ...

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I am sorry to hear you are experiencing problems with our antibody.
Could you please also let me know the following details:
1) Where did you see the bands (you can also attach a WB image)?
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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (WT MDA MB231)
Loading amount 20 µg
Specification WT MDA MB231
Gel Running Conditions Non-reduced Non-Denaturing (Native) (10% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Abcam user community

Verified customer

Submitted Sep 09 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Lewis lung carcinoma)
Loading amount 10 µg
Specification Lewis lung carcinoma
Gel Running Conditions Non-reduced Non-Denaturing (Native) (4-20%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: rt°C
Username

Abcam user community

Verified customer

Submitted Aug 18 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"