Overview

  • Product nameAnti-ST7 antibody
    See all ST7 primary antibodies
  • Description
    Rabbit polyclonal to ST7
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: PQARISAAHE ALEINEIRSR VEVPLIASST IWEIKLLPKC ATAY, corresponding to amino acids of Human ST7.

  • Positive control
    • RT 4 and U 251 cell line lysates; Human heart muscle tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122459 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

WB 1/250 - 1/500.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionMay act as a tumor suppressor.
  • Tissue specificityUbiquitously expressed, with highest levels in heart, liver and pancreas.
  • Sequence similaritiesBelongs to the ST7 family.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ETS7q antibody
    • FAM4A antibody
    • FAM4A1 antibody
    • Family with sequence similarity 4, subfamily A, member 1 antibody
    • HELG antibody
    • Protein FAM4A1 antibody
    • Protein HELG antibody
    • RAY1 antibody
    • SEN4 antibody
    • ST7 antibody
    • ST7_HUMAN antibody
    • Suppression of tumorigenicity 7 (breast) antibody
    • Suppression of tumorigenicity 7 antibody
    • Suppressor of tumorigenicity 7 protein antibody
    • TSG7 antibody
    see all

Anti-ST7 antibody images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli. Recommended concentration of ab122459 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-ST7 antibody (ab122459) at 1/250 dilution

    Lane 1 : RT 4 cell line lysate
    Lane 2 : U 251 MG cell line lysate
    Lane 3 : Human plasma
    Lane 4 : Human liver tissue lysate
    Lane 5 : Human tonsil tissue lysate
  • ab122459, at 1/500, staining ST7 in paraffin embedded Human heart muscle tissue by Immunohistochemistry.
  • Immunohistochemical staining of Human pancreas shows moderate membranous positivity using ab122459

References for Anti-ST7 antibody (ab122459)

ab122459 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"