Overview

  • Product name
  • Description
    Rabbit polyclonal to STAM1
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 147-362 of Human STAM (UniProt Q92783).

  • Positive control
    • Jurkat, Raji, K562 and NCI-H929 whole cell lysates; Human colon carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab155527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 59 kDa.
IHC-P 1/100 - 1/1000.

Target

  • Function
    Involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. May also play a role in T-cell development. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with HGS (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes.
  • Tissue specificity
    Ubiquitously expressed.
  • Sequence similarities
    Belongs to the STAM family.
    Contains 1 ITAM domain.
    Contains 1 SH3 domain.
    Contains 1 UIM (ubiquitin-interacting motif) repeat.
    Contains 1 VHS domain.
  • Post-translational
    modifications
    Phosphorylated on Tyr-198. Phosphorylated in response to IL2, IL3, IL4, IL7, CSF2/GM-CSF, EGF and PDGFB. Phosphorylated by activated PDGFRB.
  • Cellular localization
    Cytoplasm. Early endosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • HSE1 homolog antibody
    • Signal transducing adapter molecule 1 antibody
    • signal transducing adaptor molecule (SH3 domain and ITAM motif) 1 antibody
    • STAM antibody
    • STAM-1 antibody
    • STAM1_HUMAN antibody
    see all

Images

  • All lanes : Anti-STAM1 antibody (ab155527) at 1/1000 dilution

    Lane 1 : Jurkat whole cell lysate
    Lane 2 : Raji whole cell lysate
    Lane 3 : K562 whole cell lysate
    Lane 4 : NCI-H929 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 59 kDa
  • Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling STAM with ab155527 at 1/500 dilution.

References

ab155527 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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