The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
FunctionInvolved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. May also play a role in T-cell development. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with HGS (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes.
DomainThe VHS and UIM domains mediate the interaction with ubiquitinated proteins. The SH3 domain mediates the interaction with USP8. Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain.
Post-translational modificationsPhosphorylated in response to IL-2, GM-CSF, EGF and PDGF.
Cellular localizationCytoplasm. Early endosome membrane.
Signal transducing adaptor molecule (SH3 domain and ITAM motif) 2 antibody
Signal transducing adaptor molecule 2A antibody
Signal transducing adaptor molecule 2B antibody
Signal transducing adaptor molecule antibody
STAM like protein containing SH3 and ITAM domains 2 antibody
Anti-STAM2 antibody images
Western blot - Anti-STAM2 antibody (ab63372)
All lanes : Anti-STAM2 antibody (ab63372) at 1/500 dilution
Lane 1 : NIH/3T3 cell extracts
treated with EGF (200ng/ml, 30mins) Lane 2 : NIH/3T3 cell extracts
treated with EGF (200ng/ml, 30mins) with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 58 kDa Observed band size : 58 kDa Additional bands at : 38 kDa,>117 kDa. We are unsure as to the identity of these extra bands.
References for Anti-STAM2 antibody (ab63372)
This product has been referenced in:
Kapuralin K et al. STAM2, a member of the endosome-associated complex ESCRT-0 is highly expressed in neurons. Mol Cell Neurosci67:104-15 (2015).
WB, IHC-FoFr, ICC/IF
Read more (PubMed: 26101075) »