Recombinant
RabMAb

Anti-STAM2 antibody [EPR8688] (ab151545)

Overview

  • Product name
    Anti-STAM2 antibody [EPR8688]
    See all STAM2 primary antibodies
  • Description
    Rabbit monoclonal [EPR8688] to STAM2
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

  • Positive control
    • HeLa, 293T and HepG2 cell lysates; Human uterus tissue; HepG2 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20ºC.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR8688
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab151545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 58 kDa.
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/1000 - 1/10000. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. May also play a role in T-cell development. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with HGS (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes.
    • Tissue specificity
      Ubiquitously expressed.
    • Sequence similarities
      Belongs to the STAM family.
      Contains 1 ITAM domain.
      Contains 1 SH3 domain.
      Contains 1 UIM (ubiquitin-interacting motif) repeat.
      Contains 1 VHS domain.
    • Domain
      The VHS and UIM domains mediate the interaction with ubiquitinated proteins.
      The SH3 domain mediates the interaction with USP8.
      Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain.
    • Post-translational
      modifications
      Phosphorylated in response to IL-2, GM-CSF, EGF and PDGF.
    • Cellular localization
      Cytoplasm. Early endosome membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • DKFZp564C047 antibody
      • Hbp antibody
      • Hrs-binding protein antibody
      • HSE1 homolog antibody
      • Signal transducing adapter molecule 2 antibody
      • Signal transducing adaptor molecule (SH3 domain and ITAM motif) 2 antibody
      • Signal transducing adaptor molecule 2A antibody
      • Signal transducing adaptor molecule 2B antibody
      • Signal transducing adaptor molecule antibody
      • STAM like protein containing SH3 and ITAM domains 2 antibody
      • STAM-2 antibody
      • Stam2 antibody
      • STAM2_HUMAN antibody
      • STAM2A antibody
      • STAM2B antibody
      see all

    Images

    • Overlay histogram showing HeLa cells stained with ab151545 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab151545, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
    • All lanes : Anti-STAM2 antibody [EPR8688] (ab151545) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 58 kDa
    • Immunohistochemical analysis of paraffin-embedded Human uterus tissue labeling STAM2 with ab151545 at 1/50 dilution.
    • Immunofluorescent analysis of HepG2 cells labeling STAM2 with ab151545 at 1/100 dilution.

    References

    ab151545 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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