The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIHC-P: Use at a concentration of 5 µg/ml.
Antigen retrieval in 10mM citric acid for 4x5 min at 620W in a microwave oven.
Is unsuitable for WB.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionStimulates renal phosphate reabsorption, and could therefore prevent hypercalcemia.
Tissue specificityExpressed in most tissues, with the highest levels in ovary, prostate, heart, kidney and thyroid. In the kidney, expression is confined to the nephron, specifically in the distal convoluted tubule and in the collecting tubule. Not detected in the brain, liver, spleen, peripheral blood leukocytes and adrenal medulla.
Sequence similaritiesBelongs to the stanniocalcin family.