Overview

  • Product nameAnti-Stanniocalcin 1 antibody
    See all Stanniocalcin 1 primary antibodies
  • Description
    Rabbit polyclonal to Stanniocalcin 1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (LQNSAVLLVL VISASATHEA EQNDSVSPRK SRVAAQNSAE VVRCLNSALQ) of Human Stanniocalcin 1 (NP_003146).

  • Positive control
    • 721_B cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab83065 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Anti-Stanniocalcin 1 antibody images

  • Anti-Stanniocalcin 1 antibody (ab83065) at 1 µg/ml (5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 28 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)

References for Anti-Stanniocalcin 1 antibody (ab83065)

This product has been referenced in:
  • Waszak P  et al. Preconditioning enhances the paracrine effect of mesenchymal stem cells in preventing oxygen-induced neonatal lung injury in rats. Stem Cells Dev 21:2789-97 (2012). WB ; Rat . Read more (PubMed: 22533467) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing (12%)
Sample Mouse Cell lysate - whole cell (293T)
Specification 293T
Treatment transiently transfected
Blocking step BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Eric Routh

Verified customer

Submitted Mar 26 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"