The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Detects a band of approximately 31-35 kDa (predicted molecular weight: 28 kDa).
1/10 - 1/100.
Is unsuitable for ICC/IF or IHC-P.
Stimulates renal phosphate reabsorption, and could therefore prevent hypercalcemia.
Expressed in most tissues, with the highest levels in ovary, prostate, heart, kidney and thyroid. In the kidney, expression is confined to the nephron, specifically in the distal convoluted tubule and in the collecting tubule. Not detected in the brain, liver, spleen, peripheral blood leukocytes and adrenal medulla.