Overview

  • Product name
  • Description
    Goat polyclonal to STAP2
  • Specificity
    Expected to recognise both reported isoforms (NP_060190.2 and NP_001013863.1).
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide:

    ELQKKLEKRRALEH

    from the C-terminal region of Human STAP2 (100% identity for both NP_060190.2 and NP_001013863.1)

  • Positive control
    • Human heart lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab77191 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.05 - 0.3 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 50 kDa).
IHC-P Use a concentration of 1 - 2 µg/ml.

Target

  • Function
    Substrate of protein kinase PTK6. May play a regulatory role in the acute-phase response in systemic inflammation and may modulate STAT3 activity.
  • Tissue specificity
    Widely expressed.
  • Sequence similarities
    Contains 1 PH domain.
    Contains 1 SH2 domain.
  • Post-translational
    modifications
    Phosphorylated on tyrosine. Tyr-250 may be important for interaction with kinases. Tyr-22 and Tyr-322 appears to be phosphorylated by SRC.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • BKS antibody
    • Breast tumor kinase substrate antibody
    • Brk kinase substrate antibody
    • BRK substrate antibody
    • Signal transducing adaptor protein 2 antibody
    • Signal-transducing adaptor protein 2 antibody
    • Ssignal transducing adaptor family member 2 antibody
    • STAP 2 antibody
    • STAP-2 antibody
    • STAP2 antibody
    • STAP2_HUMAN antibody
    see all

Images

  • Anti-STAP2 antibody (ab77191) at 0.03 µg/ml + Human heart lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 50 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 hour
  • ab77191 at 3.8 µg/ml staining STAP2 in Human cortex by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

References

ab77191 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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