Anti-STAP2 antibody (ab77191)

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Product name

Anti-STAP2 antibody
See all STAP2 primary antibodies
Description

Goat polyclonal to STAP2
Host species

Goat
Specificity

Expected to recognise both reported isoforms (NP_060190.2 and NP_001013863.1).
Tested applications

Suitable for: WB, IHC-Pmore details
Species reactivity

Reacts with: Human
Predicted to work with: Mouse, Rat
Immunogen

Synthetic peptide:
ELQKKLEKRRALEH
from the C-terminal region of Human STAP2 (100% identity for both NP_060190.2 and NP_001013863.1)

Run BLAST with Run BLAST with
Positive control
- Human heart lysate

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration information loading...
Purity

Immunogen affinity purified
Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality

Polyclonal
Isotype

IgG
Research areas

Our Abpromise guarantee covers the use of ab77191 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		Use a concentration of 0.05 - 0.3 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 50 kDa).
IHC-P		Use a concentration of 1 - 2 µg/ml.

Function

Substrate of protein kinase PTK6. May play a regulatory role in the acute-phase response in systemic inflammation and may modulate STAT3 activity.
Tissue specificity

Widely expressed.
Sequence similarities

Contains 1 PH domain.
Contains 1 SH2 domain.
Post-translational
modifications

Phosphorylated on tyrosine. Tyr-250 may be important for interaction with kinases. Tyr-22 and Tyr-322 appears to be phosphorylated by SRC.
Cellular localization

Cytoplasm.
Target information above from: UniProt accession Q9UGK3 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 55620 Human
- Entrez Gene: 106766 Mouse
- Entrez Gene: 363334 Rat
- Omim: 607881 Human
- SwissProt: Q9UGK3 Human
- SwissProt: Q8R0L1 Mouse
- Unigene: 194385 Human
- Unigene: 204991 Mouse
- Unigene: 19078 Rat
see all
Alternative names
- BKS antibody
- Breast tumor kinase substrate antibody
- Brk kinase substrate antibody
- BRK substrate antibody
- Signal transducing adaptor protein 2 antibody
- Signal-transducing adaptor protein 2 antibody
- Ssignal transducing adaptor family member 2 antibody
- STAP 2 antibody
- STAP-2 antibody
- STAP2 antibody
- STAP2_HUMAN antibody
see all

Western blot - Anti-STAP2 antibody (ab77191)

Anti-STAP2 antibody (ab77191) at 0.03 µg/ml + Human heart lysate in RIPA buffer at 35 µg

Developed using the ECL technique.

Predicted band size: 50 kDa
Observed band size: 48 kDa (why is the actual band size different from the predicted?)

Exposure time: 1 hour
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAP2 antibody (ab77191)

ab77191 at 3.8 µg/ml staining STAP2 in Human cortex by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

ab77191 has not yet been referenced specifically in any publications.

Publishing research using ab77191? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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