Anti-Staphylococcus alpha Hemolysin antibody (ab15948)




Our Abpromise guarantee covers the use of ab15948 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 µg/ml.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceStaphylococcus aureus alpha Hemolysin binds to the membrane of eukaryotic cells resulting in the release of low-molecular weight molecules and leading to an eventual osmotic lysis. The toxin self-assembles to form first, a nonlytic oligomeric intermediate, and then, a mushroom-shaped homoheptamer structure. Heptamer oligomerization and pore formation is required for lytic activity.
    • Cellular localizationSecreted
    • Database links
      • Alternative names
        • Alpha hemolysin antibody
        • Alpha HL antibody
        • Alpha toxin antibody
        • Hla antibody
        • Hly antibody
        • Staphylococcus alpha HL antibody
        • Staphylococcus alpha toxin antibody
        see all

      Anti-Staphylococcus alpha Hemolysin antibody images

      • All lanes : Anti-Staphylococcus alpha Hemolysin antibody (ab15948) at 1 µg/ml

        Lane 1 : Positive control (purified Hla (100ng/ml))
        Lane 2 : Skin from healthy subjects
        Lane 3 : Skin from patient with chronic AD (atopic dermatitis)
        Lane 4 : Skin from patient with acute AD (atopic dermatitis)
        Lane 5 : Skin from patient with Psoriasis

        Lysates/proteins at 10 µg per lane.

        HRP conjugated donkey anti-sheep polyclonal at 1/2000 dilution

        Predicted band size : 20 kDa
        Observed band size : 33 kDa (why is the actual band size different from the predicted?)

        Image courtesy of an anonymous Abreview.

        See Abreview

      References for Anti-Staphylococcus alpha Hemolysin antibody (ab15948)

      This product has been referenced in:
      • Swofford CA  et al. Quorum-sensing Salmonella selectively trigger protein expression within tumors. Proc Natl Acad Sci U S A 112:3457-62 (2015). Read more (PubMed: 25737556) »
      • O'Rourke JP  et al. Development of a mimotope vaccine targeting the Staphylococcus aureus quorum sensing pathway. PLoS One 9:e111198 (2014). WB ; Mouse . Read more (PubMed: 25379726) »

      See all 4 Publications for this product

      Product Wall

      Application ELISA
      Sample Staphylococcus aureus Cell (different dilutions of S. aureus alpha toxin (Invi)
      Specification different dilutions of S. aureus alpha toxin (Invi
      Type Sandwich (Capture)
      Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

      Abcam user community

      Verified customer

      Submitted Oct 31 2013

      Thank you very much for your patience. I am sorry that you experiencing problems with this two antibodies.

      I am still waiting for some QC results from the lab and I will forward this to you as soon as I recive the answer.

      Every appli...

      Read More

      Thank you for contacting us.

      In order to help you with these antibodies I would like to get some more information about the experiment.

      1. Do I understand it right that the performed experiment is a sandwich ELISA? Do you mean the ab1...

      Read More
      Application Western blot
      Sample Human Tissue lysate - whole (skin biopsie)
      Loading amount 10 µg
      Specification skin biopsie
      Treatment see below (additional notes)
      Gel Running Conditions Reduced Non-Denaturing (Native) (5-16% gels)
      Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

      Abcam user community

      Verified customer

      Submitted Feb 03 2012

      Thank you for your inquiry and I apologize for the delay in this reply. I just heard back from the lab with their recommendations. They said that it is most likely the phosphate buffer that has crystallized and come out of solution, but the antibod...

      Read More