The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: Use at an assay dependent dilution.
WB: Use at a concentration of 1 µg/ml.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceStaphylococcus aureus alpha Hemolysin binds to the membrane of eukaryotic cells resulting in the release of low-molecular weight molecules and leading to an eventual osmotic lysis. The toxin self-assembles to form first, a nonlytic oligomeric intermediate, and then, a mushroom-shaped homoheptamer structure. Heptamer oligomerization and pore formation is required for lytic activity.
Western blot - Anti-Staphylococcus alpha Hemolysin antibody (ab15948)
All lanes : Anti-Staphylococcus alpha Hemolysin antibody (ab15948) at 1 µg/ml
Lane 1 : Positive control (purified Hla (100ng/ml)) Lane 2 : Skin from healthy subjects Lane 3 : Skin from patient with chronic AD (atopic dermatitis) Lane 4 : Skin from patient with acute AD (atopic dermatitis) Lane 5 : Skin from patient with Psoriasis
Lysates/proteins at 10 µg per lane.
Secondary HRP conjugated donkey anti-sheep polyclonal at 1/2000 dilution