Anti-Staphylococcus alpha Hemolysin antibody (HRP) (ab15949)

Overview

  • Product nameAnti-Staphylococcus alpha Hemolysin antibody (HRP)
    See all Staphylococcus alpha Hemolysin primary antibodies
  • Description
    Sheep polyclonal to Staphylococcus alpha Hemolysin (HRP)
  • ConjugationHRP
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Staphylococcus aureus
  • Immunogen

    Highly pure (> 95%) Staphylococcus aureus alpha Hemolysin

Properties

Applications

Our Abpromise guarantee covers the use of ab15949 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notesELISA: 1/300. This dilution will give an absorbance of >1.0 for a 10 ng/ml standard after a five minute incubation.
    WB: 1/1000. Predicted molecular weight: 35.9 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceStaphylococcus aureus alpha Hemolysin binds to the membrane of eukaryotic cells resulting in the release of low-molecular weight molecules and leading to an eventual osmotic lysis. The toxin self-assembles to form first, a nonlytic oligomeric intermediate, and then, a mushroom-shaped homoheptamer structure. Heptamer oligomerization and pore formation is required for lytic activity.
    • Cellular localizationSecreted
    • Database links
      • Alternative names
        • Alpha hemolysin antibody
        • Alpha HL antibody
        • Alpha toxin antibody
        • Hla antibody
        • Hly antibody
        • Staphylococcus alpha HL antibody
        • Staphylococcus alpha toxin antibody
        see all

      References for Anti-Staphylococcus alpha Hemolysin antibody (HRP) (ab15949)

      This product has been referenced in:
      • Khodaverdian V  et al. Discovery of antivirulence agents against methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother 57:3645-52 (2013). Read more (PubMed: 23689713) »
      • Meredith TC  et al. Late-stage polyribitol phosphate wall teichoic acid biosynthesis in Staphylococcus aureus. J Bacteriol 190:3046-56 (2008). Read more (PubMed: 18281399) »

      See all 3 Publications for this product

      Product Wall

      I am still waiting for a reply from the lab regarding the exact protocol used for WB validation. In the mean time, this antibody has been used in WB in the following references:

      http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2088803/?tool=pubmed...

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"