Anti-Staphylococcus aureus antibody [704] (ab37644)


  • Product nameAnti-Staphylococcus aureus antibody [704]
    See all Staphylococcus aureus primary antibodies
  • Description
    Mouse monoclonal [704] to Staphylococcus aureus
  • Tested applicationsSuitable for: ELISA, ICC/IF, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with Staphylococcus aureus, Protein A-negative Staphylococcus aureus and Staphylococcus epidermidis. Does not cross-react with Corynebacteria species, Streptococcus species or Mycoplasma species.
  • Immunogen

    UV-inactivated Staphylococcus aureus cells.



Our Abpromise guarantee covers the use of ab37644 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration. May be used to detect and quantify Staphylococcus aureus cells.
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. PubMed: 21208278
Flow Cyt Use at an assay dependent concentration. PubMed: 20016671ab18392-Mouse monoclonal IgG3, is suitable for use as an isotype control with this antibody.


  • RelevanceS.aureus binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface, which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.
  • Cellular localizationSecreted; cell wall.
  • Alternative names
    • IgG binding protein A antibody
    • Immunoglobulin G binding protein A antibody
    • SPA antibody

Anti-Staphylococcus aureus antibody [704] images

  • ab37644 staining Staphylococcus aureus in 16HBE cells by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed with 4% paraformaldehyde, blocked with 5% normal donkey serum, and stained with ab37644. The secondary used was an anti-mouse IgG Alexa Fluor 488 (green). Phalloidin (blue).

    Scale bar 20 µm.

References for Anti-Staphylococcus aureus antibody [704] (ab37644)

This product has been referenced in:
  • Chen J  et al. [(99m)Tc]cFLFLF for Early Diagnosis and Therapeutic Evaluation in a Rat Model of Acute Osteomyelitis. Mol Imaging Biol 17:337-44 (2015). Read more (PubMed: 25323104) »
  • Johansen LK  et al. A porcine model of acute, haematogenous, localized osteomyelitis due to Staphylococcus aureus: a pathomorphological study. APMIS 119:111-8 (2011). IHC-P . Read more (PubMed: 21208278) »

See all 5 Publications for this product

Product Wall

Application Immunocytochemistry
Sample Mouse Cultured Cells (Mast Cells (HMC-1))
Permeabilization Yes - Triton X-100
Specification Mast Cells (HMC-1)
Blocking step BSA as blocking agent for 60 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative Formaldehyde

Dr. Tim Biggs

Verified customer

Submitted Jun 15 2016

Ab20002 - Mouse monoclonal [702] to Staphylococcus aureus and Ab37644 - Mouse monoclonal [704] to Staphylococcus aureus recognize peptidoglycan which is a cell wall component, so, by definition, this is on the surface of the cells.

About the...

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Thank you for your enquiry.

I havecontacted the source for all the Staphylococcus aureus antibodies we have that have been tested in WB and/or ELISA to ask if they have been tested for crossreactivity. Here is the information:


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Thank you for contacting Abcam

The antibody, ab37644 is covered under our Abpromise for six months and is guaranteed to work in ICC/IF. If we cannot resolve the issue you are having with the antibody then I would be happy to either send a repl...

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Thank you for contacting Abcam.

The antibody ab37644 recognizes an epitope on the peptidoglycan. The epitope recognized is not on Protein A.

I hope that this helps clarify matters. If there is anything else I can help you with, plea...

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Thank you for calling Abcam earlier.

I have attached our protocol for mouse on mouse staining and the link to our EasyLink kits is below:

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Thank you for contacting us. Staph aureus ab37644, recognizes an epitope on the peptidoglycan. A more detailed location has not been mapped. The immunogen is UV-inactivated Staphylococcus aureus cells. I hope this information is helpful to you. Pl...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (THP-1 Macrophages)
Specification THP-1 Macrophages
Fixative No fixative - LIVE cells
Permeabilization No

Abcam user community

Verified customer

Submitted Jul 26 2007