Anti-Staphylococcus aureus antibody (ab73962)

Overview

  • Product name
    Anti-Staphylococcus aureus antibody
    See all Staphylococcus aureus primary antibodies
  • Description
    Chicken polyclonal to Staphylococcus aureus
  • Specificity
    This antibody is recognizing both, native and recombinant Protein A, from various sources. Product has not been analyzed strain-specifically.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Staphylococcus aureus
  • Immunogen

    Purified full length recombinant Staphylococcal Protein A

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: 0.9% Sodium chloride
  • Concentration information loading...
  • Purity
    IgY fraction
  • Purification notes
    The IgY fraction is isolated by a two step PEG precipitation procedure followed by ammonium sulphate precipitation.
  • Clonality
    Polyclonal
  • Isotype
    IgY
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab73962 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    ELISA: 1/5000.
    WB: 1/5000. Predicted molecular weight: 60 kDa.

    One precipitin arc in immunodiffusion against Staphylococcal Protein A.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      S.aureus binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface, which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.
    • Cellular localization
      Secreted; cell wall.
    • Database links
      • Alternative names
        • IgG binding protein A antibody
        • Immunoglobulin G binding protein A antibody
        • SPA antibody

      References for Anti-Staphylococcus aureus antibody (ab73962)

      ab73962 has not yet been referenced specifically in any publications.

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      Thank you for your enquiry.

      I havecontacted the source for all the Staphylococcus aureus antibodies we have that have been tested in WB and/or ELISA to ask if they have been tested for crossreactivity. Here is the information:

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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