Overview

  • Product name
    Anti-Staphylococcus Enterotoxin A antibody
    See all Staphylococcus Enterotoxin A primary antibodies
  • Description
    Rabbit polyclonal to Staphylococcus Enterotoxin A
  • Specificity
    Ab15897 gives absorbance readings (410 nm) of less than 0.100 for 10 ng/ml preparations of staphylococcal enterotoxins B through E, ET, TSST, and alpha hemolysin. Cross reacts with Staphylococcus Enterotoxin E
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Staphylococcus aureus
  • Immunogen

    Highly purified (> 95%) Staphylococcus aureus Enterotoxin A

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: None
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab15897 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    ELISA: Use at a concentration of 10 µg/ml. When a standard well is coated at this concentration (100µl/well) the following results are obtained:
    10 ng/ml SEA, Absorbance (410 nm) = 2.00 after 15 minute incubation.
    1.25 ng/ml SEA, Absorbance (410 nm) = 0.78 after 15 minute incubation.
    WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 30 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Staphylococcal enterotoxins represent a group of proteins, which are secreted by Staphylococcus aureus and cause the intoxication staphylococcal food poisoning syndrome. The illness characterised by high fever, hypotension, diarrhea, shock, and in some cases death. Their molecular masses range between 27 and 30 kDa. At present, seven enterotoxins are known, namely A, B, C1, C2, C3, D and E. Their amino acid sequences have been determined and it was shown that all are single chain polypeptides containing one disulfide bond formed by two half cystines located in the middle of the polypeptide chain, which form the so called cysteine loop. Enterotoxins are known to be most potent T cell mitogens. T cell activation accompanied by induction of interleukin 2 and interferon is conditioned by high affinity interaction of S.enterotoxins with class II main histocompatibility complex (MHC) molecules and subsequent presentation of the complex formed to a variable region of the T cell receptor.
    • Cellular localization
      Secreted
    • Database links
      • Alternative names
        • EntA antibody
        • Enterotoxin A antibody
        • Enterotoxin type A antibody
        • SEA antibody
        • Staphylococcus enterotoxin type A antibody
        see all

      References for Anti-Staphylococcus Enterotoxin A antibody (ab15897)

      This product has been referenced in:
      • Niu X  et al. Combining Single Strand Oligodeoxynucleotides and CRISPR/Cas9 to Correct Gene Mutations in ß-Thalassemia-induced Pluripotent Stem Cells. J Biol Chem 291:16576-85 (2016). ICC/IF ; Human . Read more (PubMed: 27288406) »

      See 1 Publication for this product

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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