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Full length protein corresponding to Staphylococcus Enterotoxin B.
Database link: P01552
Our Abpromise guarantee covers the use of ab190465 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Neutralising||Use at an assay dependent concentration.
10 nM of ab190465 completely inhibits superantigenic activity of 0.1 ng/ml SEB.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 31 kDa.|
|Sandwich ELISA||Use a concentration of 1 µg/ml.
ab190465 can be used for capture of SEB in an ELISA sandwich assay.
ab190337 can be used as detection antibody, along with another anti rabbit HRP antibody.
Toxin Neutralization: dose dependent suppression of Staphylococcus Enterotoxin B-induced IFNγ production in Human peripheral mononuclear cells (PBMC).
Capture ELISA in which ab190465 was coated to the plate overnight at 4oC at a concentration of 1 μg/mL. Staphylococcus Enterotoxin B was serially diluted semi-log (1:3.16 serial dilutions) from 20 ng/mL and incubated on the antibody coated plate. Captured Staphylococcus Enterotoxin B was detected using an anti-Staphylococcus Enterotoxin B polyclonal antibody and anti-rabbit conjugate HRP conjugate. Data represent three different curves detected across the plate.
ab190465 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"