Anti-Staphylococcus Enterotoxin B antibody [3D10] (ab190465)

Overview

  • Product name
    Anti-Staphylococcus Enterotoxin B antibody [3D10]
    See all Staphylococcus Enterotoxin B primary antibodies
  • Description
    Mouse monoclonal [3D10] to Staphylococcus Enterotoxin B
  • Host species
    Mouse
  • Specificity
    ab190465 does not appear to cross react with TSST-1 or SEC-1.
  • Tested applications
    Suitable for: Neutralising, WB, Sandwich ELISAmore details
    Unsuitable for: ELISA
  • Species reactivity
    Reacts with: Staphylococcus aureus
  • Immunogen

    Full length protein corresponding to Staphylococcus Enterotoxin B.
    Database link: P01552

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Constituent: 100% PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    3D10
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab190465 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Neutralising Use at an assay dependent concentration.

10 nM of ab190465 completely inhibits superantigenic activity of 0.1 ng/ml SEB.

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 31 kDa.
Sandwich ELISA Use a concentration of 1 µg/ml.

ab190465 can be used for capture of SEB in an ELISA sandwich assay.

ab190337 can be used as detection antibody, along with another anti rabbit HRP antibody.

  • Application notes
    Is unsuitable for ELISA.
  • Target

    • Relevance
      Staphylococcal enterotoxins represent a group of proteins, which are secreted by Staphylococcus aureus and cause staphylococcal food poisoning syndrome. The illness is characterised by high fever, hypotension, diarrhea, shock, and in some cases death. Their molecular masses range between 27 and 30 kDa. At present, seven enterotoxins are known, namely A, B, C1, C2, C3, D and E. Their amino acid sequences have been determined and it was shown that all are single chain polypeptides containing one disulfide bond formed by two half cystines located in the middle of the polypeptide chain, which form the so called cysteine loop. SEB is an extremely potent activator of T cells, stimulating the production and secretion of various cytokines which mediate many of the toxic effects of SEB.
    • Cellular localization
      Secreted
    • Database links
      • Alternative names
        • Ent B antibody
        • EntB antibody
        • Enterotoxin type B antibody
        • SEB antibody
        • Staphylococcal enterotoxin B antibody
        see all

      Images

      • Toxin Neutralization: dose dependent suppression of Staphylococcus Enterotoxin B-induced IFNγ production in Human peripheral mononuclear cells (PBMC).

      • Capture ELISA in which ab190465 was coated to the plate overnight at 4oC at a concentration of 1 μg/mL. Staphylococcus Enterotoxin B was serially diluted semi-log (1:3.16 serial dilutions) from 20 ng/mL and incubated on the antibody coated plate. Captured Staphylococcus Enterotoxin B was detected using an anti-Staphylococcus Enterotoxin B polyclonal antibody and anti-rabbit conjugate HRP conjugate. Data represent three different curves detected across the plate.

      • Anti-Staphylococcus Enterotoxin B antibody [3D10] (ab190465) at 1 µg/ml + Staphylococcus Enterotoxin B at 0.05 µg

        Secondary
        anti-mouse HRP conjugate.

        Predicted band size: 31 kDa

      References

      ab190465 has not yet been referenced specifically in any publications.

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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