Anti-Staphylococcus Enterotoxin B antibody (ab15919)

Overview

  • Product name
    Anti-Staphylococcus Enterotoxin B antibody
    See all Staphylococcus Enterotoxin B primary antibodies
  • Description
    Sheep polyclonal to Staphylococcus Enterotoxin B
  • Specificity
    Absorbance readings (410 nm) of less than 0.100 for 10 ng/ml preparations of other staphylococcal enterotoxins (of the B through E set), TSST, ET, and alpha hemolysin.
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with enterotoxin B.
  • Immunogen

    Purified toxin (>95% pure).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: None
    Constituents: 0.9% Sodium chloride, 0.01M PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab15919 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    ELISA: Use at a concentration of 10 µg/ml.
    At 100 µl/well, the following results are obtained:
    10 ng/ml SEA Abs (410 nm) = 2.00 after 15 minute incubation.
    1.25 ng/ml SEA Abs (410 nm) = 0.78 after 15 minute incubation.
    WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 32 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Staphylococcal enterotoxins represent a group of proteins, which are secreted by Staphylococcus aureus and cause staphylococcal food poisoning syndrome. The illness is characterised by high fever, hypotension, diarrhea, shock, and in some cases death. Their molecular masses range between 27 and 30 kDa. At present, seven enterotoxins are known, namely A, B, C1, C2, C3, D and E. Their amino acid sequences have been determined and it was shown that all are single chain polypeptides containing one disulfide bond formed by two half cystines located in the middle of the polypeptide chain, which form the so called cysteine loop. SEB is an extremely potent activator of T cells, stimulating the production and secretion of various cytokines which mediate many of the toxic effects of SEB.
    • Cellular localization
      Secreted
    • Alternative names
      • Ent B antibody
      • EntB antibody
      • Enterotoxin type B antibody
      • SEB antibody
      • Staphylococcal enterotoxin B antibody
      see all

    References

    ab15919 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    I am sorry to inform you, but please be aware that our products are for research only and not suitable to be used as diagnostic tools. This information can be found in our Terms and Conditions under Product use l...

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    I can confirm that ab15919 Staphylococcus Enterotoxin B antibody is an affinity purified antibody that is specific for staphylococcal Enterotoxin B (SEB). I am sorry it has not been tested for detection of the S. ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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