The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: Use at a concentration of 10 µg/ml. When a standard well is coated at this concentration (100µl/well) the following results are obtained:
10 ng/ml Staphylococcus aureus Enterotoxin D, Absorbance (410 nm) = 2.00 after 15 minute incubation.
1.25 ng/ml Staphylococcus aureus Enterotoxin D, Absorbance (410 nm) = 0.78 after 15 minute incubation.
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 30 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceStaphylococcal enterotoxins represent a group of proteins, which are secreted by Staphylococcus aureus and cause the intoxication staphylococcal food poisoning syndrome. The illness is characterised by high fever, hypotension, diarrhea, shock, and in some cases death. Their molecular masses range between 27 and 30 kDa. At present, seven enterotoxins are known, namely A, B, C (subtypes C1, C2, C3), D and E. Their amino acid sequences have been determined and it was shown that all are single chain polypeptides containing one disulfide bond formed by two half cystines located in the middle of the polypeptide chain, which form the so called cysteine loop. Enterotoxins are known to be most potent T cell mitogens. T cell activation accompanied by induction of interleukin 2 and interferon is conditioned by high affinity interaction of S.enterotoxins with class II main histocompatibility complex (MHC) molecules and subsequent presentation of the complex formed to a variable region of the T cell receptor.