Anti-Staphylococcus Enterotoxin D antibody (Biotin) (ab15913)

Overview

  • Product name
    Anti-Staphylococcus Enterotoxin D antibody (Biotin)
    See all Staphylococcus Enterotoxin D primary antibodies
  • Description
    Rabbit polyclonal to Staphylococcus Enterotoxin D (Biotin)
  • Conjugation
    Biotin
  • Specificity
    Absorbance readings (410 nm) of less than 0.100 for 10 ng/ml preparations of other staphylococcal enterotoxins (of the B through E set), TSST, ET, and alpha hemolysin.
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with Staphylococcus enterotoxin D.
  • Immunogen

    Purified toxin (>95% pure).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: None
    Constituents: 1% BSA, 0.01M PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab15913 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    ELISA: 1/300.
    This is an approximate working dilution for use in a sandwich ELISA. This dilution will give an absorbance of >1.0 for a 10 ng standard after a 5 minute incubation.
    WB: 1/1000. Predicted molecular weight: 31 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Staphylococcal enterotoxins represent a group of proteins, which are secreted by Staphylococcus aureus and cause the intoxication staphylococcal food poisoning syndrome. The illness is characterised by high fever, hypotension, diarrhea, shock, and in some cases death. Their molecular masses range between 27 and 30 kDa. At present, seven enterotoxins are known, namely A, B, C (subtypes C1, C2, C3), D and E. Their amino acid sequences have been determined and it was shown that all are single chain polypeptides containing one disulfide bond formed by two half cystines located in the middle of the polypeptide chain, which form the so called cysteine loop. Enterotoxins are known to be most potent T cell mitogens. T cell activation accompanied by induction of interleukin 2 and interferon is conditioned by high affinity interaction of S.enterotoxins with class II main histocompatibility complex (MHC) molecules and subsequent presentation of the complex formed to a variable region of the T cell receptor.
    • Cellular localization
      Secreted
    • Alternative names
      • Ent D antibody
      • EntD antibody
      • Enterotoxin type D antibody
      • SED antibody
      • Staphylococcal enterotoxin D antibody
      • Staphylococcus enterotoxin type D antibody
      see all

    References for Anti-Staphylococcus Enterotoxin D antibody (Biotin) (ab15913)

    ab15913 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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