The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: 1/300.
This is an approximate working dilution for use in a sandwich ELISA. This dilution will give an absorbance of >1.0 for a 10 ng standard after a 5 minute incubation.
WB: 1/1000. Predicted molecular weight: 28.9 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceStaphylococcus enterotoxin H (SEH) belongs to the staphylococcal enterotoxin (SE) family of superantigens (SAgs). SEH has structural similarities to other SE; however, its biological properties are less well characterized. SEH binds with high affinity to human major histocompatibility complex (MHC) class II and exhibits strong mitogenic activity in human T cells.