Anti-StAR antibody (ab96637)
Key features and details
- Rabbit polyclonal to StAR
- Suitable for: ICC, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-StAR antibody
See all StAR primary antibodies -
Description
Rabbit polyclonal to StAR -
Host species
Rabbit -
Tested applications
Suitable for: ICC, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow, Dog, Pig -
Immunogen
Recombinant protein fragment corresponding to a region within amino acids 15 - 238 of Human StAR (NP_000340).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96637 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use at an assay dependent concentration.
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WB | (3) |
1/100 - 1/10000. Predicted molecular weight: 32 kDa.
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IHC-P |
1/100 - 1/1000.
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Notes |
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ICC
Use at an assay dependent concentration. |
WB
1/100 - 1/10000. Predicted molecular weight: 32 kDa. |
IHC-P
1/100 - 1/1000. |
Target
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Function
Plays a key role in steroid hormone synthesis by enhancing the metabolism of cholesterol into pregnenolone. Mediates the transfer of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane where it is cleaved to pregnenolone. -
Tissue specificity
Expressed in gonads, adrenal cortex and kidney. -
Pathway
Steroid metabolism; cholesterol metabolism. -
Involvement in disease
Defects in STAR are the cause of adrenal hyperplasia type 1 (AH1) [MIM:201710]. The most severe form of adrenal hyperplasia. It is a condition characterized by onset of profound adrenocortical insufficiency shortly after birth, hyperpigmentation reflecting increased production of pro-opiomelanocortin, elevated plasma renin activity as a consequence of reduced aldosterone synthesis, and male pseudohermaphroditism resulting from deficient fetal testicular testosterone synthesis. Affected individuals are phenotypic females irrespective of gonadal sex, and frequently die in infancy if mineralocorticoid and glucocorticoid replacement are not instituted. -
Sequence similarities
Contains 1 START domain. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 6770 Human
- Omim: 600617 Human
- SwissProt: P49675 Human
- SwissProt: Q28996 Pig
- Unigene: 521535 Human
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Alternative names
- Cholesterol trafficker antibody
- Luteinizing hormone induced protein antibody
- mitochondrial antibody
see all
Images
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Anti-StAR antibody (ab96637) at 1/1000 dilution + K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 32 kDa12% SDS-PAGE
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Immunohistochemical analysis of paraffin-embedded human hepatoma tissue staining StAR protein at cytosol with ab96637 at 1/500.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min. -
Immunofluorescence analysis of 4% paraformaldehyde-fixed HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate labeling StAR protein at mitochondria with ab96637 at 1/500 dilution. Blue: Hoechst 33342 staining.
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All lanes : Anti-StAR antibody (ab96637) at 1/5000 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : Transfected HEK-293T whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 32 kDa12% SDS-PAGE
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All lanes : Anti-StAR antibody (ab96637) at 1/1000 dilution
Lane 1 : Whole tissue lysate prepared from murine adrenal gland
Lane 2 : Whole tissue lysate prepared from rat adrenal gland
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP conjugated goat anti-rabbit polyclonal at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 32 kDa
Exposure time: 20 seconds
Datasheets and documents
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SDS download
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Datasheet download
References (21)
ab96637 has been referenced in 21 publications.
- Manna PR et al. Expression and Function of StAR in Cancerous and Non-Cancerous Human and Mouse Breast Tissues: New Insights into Diagnosis and Treatment of Hormone-Sensitive Breast Cancer. Int J Mol Sci 24:N/A (2023). PubMed: 36614200
- Mohr MA et al. Puberty enables oestradiol-induced progesterone synthesis in female mouse hypothalamic astrocytes. J Neuroendocrinol 34:e13082 (2022). PubMed: 35000221
- Gudelska M et al. Chemerin Affects P4 and E2 Synthesis in the Porcine Endometrium during Early Pregnancy. Int J Mol Sci 23:N/A (2022). PubMed: 35055130
- Przygrodzka E et al. PKA and AMPK Signaling Pathways Differentially Regulate Luteal Steroidogenesis. Endocrinology 162:N/A (2021). PubMed: 33502468
- Plewes MR et al. Trafficking of cholesterol from lipid droplets to mitochondria in bovine luteal cells: Acute control of progesterone synthesis. FASEB J 34:10731-10750 (2020). PubMed: 32614098