Overview

  • Product nameAnti-StAR antibody
    See all StAR primary antibodies
  • Description
    Rabbit polyclonal to StAR
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Dog, Pig
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 15 - 238 of Human StAR (NP_000340).

  • Positive control
    • 293T, A431, HeLa, HepG2, MOLT4 and Raji cell lysates

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab96637 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 32 kDa.

Target

  • FunctionPlays a key role in steroid hormone synthesis by enhancing the metabolism of cholesterol into pregnenolone. Mediates the transfer of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane where it is cleaved to pregnenolone.
  • Tissue specificityExpressed in gonads, adrenal cortex and kidney.
  • PathwaySteroid metabolism; cholesterol metabolism.
  • Involvement in diseaseDefects in STAR are the cause of adrenal hyperplasia type 1 (AH1) [MIM:201710]. The most severe form of adrenal hyperplasia. It is a condition characterized by onset of profound adrenocortical insufficiency shortly after birth, hyperpigmentation reflecting increased production of pro-opiomelanocortin, elevated plasma renin activity as a consequence of reduced aldosterone synthesis, and male pseudohermaphroditism resulting from deficient fetal testicular testosterone synthesis. Affected individuals are phenotypic females irrespective of gonadal sex, and frequently die in infancy if mineralocorticoid and glucocorticoid replacement are not instituted.
  • Sequence similaritiesContains 1 START domain.
  • Cellular localizationMitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cholesterol trafficker antibody
    • Luteinizing hormone induced protein antibody
    • mitochondrial antibody
    • Mitochondrial steroid acute regulatory protein antibody
    • StAR antibody
    • StAR related lipid transfer (START) domain containing 1 antibody
    • STAR_HUMAN antibody
    • StARD1 antibody
    • START domain containing 1 antibody
    • START domain containing protein 1 antibody
    • START domain-containing protein 1 antibody
    • Steroid acute regulatory protein antibody
    • Steroidogenic acute regulator antibody
    • Steroidogenic acute regulatory protein antibody
    • Steroidogenic acute regulatory protein mitochondrial antibody
    see all

Anti-StAR antibody images

  • Anti-StAR antibody (ab96637) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg

    Predicted band size : 32 kDa
  • All lanes : Anti-StAR antibody (ab96637) at 1/1000 dilution

    Lane 1 : Whole tissue lysate prepared from murine adrenal gland
    Lane 2 : Whole tissue lysate prepared from rat adrenal gland

    Lysates/proteins at 50 µg per lane.

    Secondary
    HRP conjugated goat anti-rabbit polyclonal at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 32 kDa


    Exposure time : 20 seconds

    Image courtesy of an anonymous Abreview.

    See Abreview

References for Anti-StAR antibody (ab96637)

This product has been referenced in:
  • Barbero-Camps E  et al. Endoplasmic reticulum stress mediates amyloid ß neurotoxicity via mitochondrial cholesterol trafficking. Am J Pathol 184:2066-81 (2014). Mouse . Read more (PubMed: 24815354) »
  • Chen C  et al. Estradiol modulates translocator protein (TSPO) and steroid acute regulatory protein (StAR) via protein kinase A (PKA) signaling in hypothalamic astrocytes. Endocrinology 155:2976-85 (2014). WB ; Rat . Read more (PubMed: 24877623) »

See all 2 Publications for this product

Product Wall

Thank you very much for your interest in ab96637. Sinceab96637 has not been tested in pig, I can offer a discount off a future purchase if you buy ab96637 now, test it in pig, and submit feedback to us in the form of an Abreview. It doesn’t matte...

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Thank you for submitting your completed questionnaire. To our knowledge ab96637 has not been previously tested in IHC-P. Thus is may be that this antibody does not function effectively in this application. However, results may be improved by doing an ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Adrenal)
Loading amount 50 µg
Specification Adrenal
Gel Running Conditions Reduced Denaturing (12%)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Aug 23 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Liver)
Loading amount 50 µg
Specification Liver
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Oct 20 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (THP-1)
Loading amount 25 µg
Specification THP-1
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Sep 29 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"