Recombinant Anti-StAR antibody [EP7639] (ab133657)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP7639] to StAR
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-StAR antibody [EP7639]
See all StAR primary antibodies -
Description
Rabbit monoclonal [EP7639] to StAR -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human StAR aa 250 to the C-terminus. The exact sequence is proprietary.
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Positive control
- Human adrenal gland lysate; Human adrenal gland tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP7639 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133657 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/10000 - 1/50000. Detects a band of approximately 30 kDa (predicted molecular weight: 32 kDa).
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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WB
1/10000 - 1/50000. Detects a band of approximately 30 kDa (predicted molecular weight: 32 kDa). |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Plays a key role in steroid hormone synthesis by enhancing the metabolism of cholesterol into pregnenolone. Mediates the transfer of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane where it is cleaved to pregnenolone. -
Tissue specificity
Expressed in gonads, adrenal cortex and kidney. -
Pathway
Steroid metabolism; cholesterol metabolism. -
Involvement in disease
Defects in STAR are the cause of adrenal hyperplasia type 1 (AH1) [MIM:201710]. The most severe form of adrenal hyperplasia. It is a condition characterized by onset of profound adrenocortical insufficiency shortly after birth, hyperpigmentation reflecting increased production of pro-opiomelanocortin, elevated plasma renin activity as a consequence of reduced aldosterone synthesis, and male pseudohermaphroditism resulting from deficient fetal testicular testosterone synthesis. Affected individuals are phenotypic females irrespective of gonadal sex, and frequently die in infancy if mineralocorticoid and glucocorticoid replacement are not instituted. -
Sequence similarities
Contains 1 START domain. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 6770 Human
- Omim: 600617 Human
- SwissProt: P49675 Human
- Unigene: 521535 Human
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Alternative names
- Cholesterol trafficker antibody
- Luteinizing hormone induced protein antibody
- mitochondrial antibody
see all
Images
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Anti-StAR antibody [EP7639] (ab133657) at 1/10000 dilution + Human adrenal gland lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 32 kDa -
Immunohistochemical analysis of paraffin-embedded Human adrenal gland tissue labelling StAR with ab133657 at 1/250 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ab133657 showing positive staining in Normal testis tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ab133657 showing positive staining in Adrenocortical carcinoma tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ab133657 showing negative staining in Skeletal muscle tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ab133657 showing positive staining in Sertoli-Leydig cell tumor tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab133657 has been referenced in 10 publications.
- Wang J et al. H2 S catalysed by CBS regulates testosterone synthesis through affecting the sulfhydrylation of PDE. J Cell Mol Med 25:3460-3468 (2021). PubMed: 33713531
- Ma J et al. Single-cell transcriptomic analysis of endometriosis provides insights into fibroblast fates and immune cell heterogeneity. Cell Biosci 11:125 (2021). PubMed: 34233737
- Bose HS et al. Regulation of Estradiol Synthesis by Aromatase Interacting Partner in Breast (AIPB). Mol Cell Biol 41:e0035721 (2021). PubMed: 34460330
- Asiabi P et al. Immunodetection and quantification of enzymatic markers in theca cells: the early process of ovarian steroidogenesis†. Biol Reprod 102:145-155 (2020). PubMed: 31504196
- Bai J et al. Discovery of methylpyrimidine ring-fused diterpenoid analogs as a novel testosterone synthesis promoter. RSC Adv 9:9709-9717 (2019). PubMed: 35520707
- Manna PR et al. Overexpression of the steroidogenic acute regulatory protein in breast cancer: Regulation by histone deacetylase inhibition. Biochem Biophys Res Commun 509:476-482 (2019). PubMed: 30595381
- Chen Y et al. Differentiation of human adipose derived stem cells into Leydig-like cells with molecular compounds. J Cell Mol Med 23:5956-5969 (2019). PubMed: 31293077
- Bai J et al. Synthesis and biological evaluation of methylpyrimidine-fused tricyclic diterpene analogs as novel oral anti-late-onset hypogonadism agents. Eur J Med Chem 176:21-40 (2019). PubMed: 31091478
- Ide H et al. Modulation of AKR1C2 by curcumin decreases testosterone production in prostate cancer. Cancer Sci 109:1230-1238 (2018). WB ; Human . PubMed: 29369461
- Guo X et al. Induced pluripotent stem cell-derived conditional medium promotes Leydig cell anti-apoptosis and proliferation via autophagy and Wnt/ß-catenin pathway. J Cell Mol Med 22:3614-3626 (2018). PubMed: 29667777