Starch Assay Kit (ab83393)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Cell Lysate, Food samples, Tissue Lysate
- Sensitivity: 4 µg/ml
Overview
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Product name
Starch Assay Kit -
Detection method
Colorimetric/Fluorometric -
Sample type
Cell Lysate, Tissue Lysate, Food samples -
Assay type
Quantitative -
Sensitivity
> 4 µg/ml -
Range
4 µg/ml - 2000 µg/ml -
Assay time
0h 40m -
Product overview
Abcam's Starch Assay Kit provides an easy, accurate assay to measure starch levels in a variety of samples. In the assay, starch is hydrolyzed to glucose which is oxidized to generate color (570 nm) and fluorescence (Ex/Em = 535/587 nm). The assay can detect starch at 0.0004 to 2 mg/ml.
Visit our FAQs page for tips and troubleshooting.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K647 Starch Colorimetric/Fluorometric Assay Kit. K647-100 is the same size as the 100 test size of ab83393.
Starch is a complex carbohydrate consisting of a large number of glucose units. All plants contain starch, present as amylose, (linear α-1,4 linked polymer) and amylopectin, (highly α-1,6 branched α-1,4 polymer). Starch generally contains 0-25% amylose and 75-100% amylopectin.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Development Buffer 1 x 25ml Development Enzyme Mix (Lyophilised) 1 vial Hydrolysis Buffer 1 x 25ml Hydrolysis Enzyme Mix (Lyophilised) 1 vial OxiRed Probe in DMSO 1 vial Starch Standard (2.0 mg/ml) 1 x 100µl -
Research areas
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Relevance
Starch is a complex carbohydrate consisting of a large number of glucose units. All plants contain starch, present as amylose, (linear alpha-1,4 linked polymer) and amylopectin, (highly alpha-1,6 branched alpha-1,4 polymer). Starch generally contains 0-25% amylose and 75–100% amylopectin. -
Alternative names
- Amylopectin
- Amylose
Images
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab83393 has been referenced in 5 publications.
- Laloum D et al. Exploring the Contribution of Autophagy to the Excess-Sucrose Response in Arabidopsis thaliana. Int J Mol Sci 23:N/A (2022). PubMed: 35409249
- Song Y et al. Regulation of Carbon Partitioning in the Seed of the Model Legume Medicago truncatula and Medicago orbicularis: A Comparative Approach. Front Plant Sci 8:2070 (2017). Functional Studies . PubMed: 29312368
- Castro PH et al. SIZ1-Dependent Post-Translational Modification by SUMO Modulates Sugar Signaling and Metabolism in Arabidopsis thaliana. Plant Cell Physiol 56:2297-311 (2015). PubMed: 26468507
- Mittal A et al. TOPOISOMERASE 6B is involved in chromatin remodelling associated with control of carbon partitioning into secondary metabolites and cell walls, and epidermal morphogenesis in Arabidopsis. J Exp Bot N/A:N/A (2014). Functional Studies . PubMed: 24821950
- Martin AR & Thomas SC Size-dependent changes in leaf and wood chemical traits in two Caribbean rainforest trees. Tree Physiol 33:1338-53 (2013). PubMed: 24336517