Recombinant Anti-STARD4 antibody [EPR17847] (ab200344)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17847] to STARD4
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-STARD4 antibody [EPR17847]
See all STARD4 primary antibodies -
Description
Rabbit monoclonal [EPR17847] to STARD4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- SH-SY5Y, HepG2, 293 and U-937 whole cell lysate. Human fetal heart, fetal kidney and fetal liver lysate. SH-SY5Y cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17847 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab200344 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000. Predicted molecular weight: 24 kDa.
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ICC/IF |
1/100.
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Notes |
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WB
1/1000. Predicted molecular weight: 24 kDa. |
ICC/IF
1/100. |
Target
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Function
May be involved in the intracellular transport of sterols or other lipids. May bind cholesterol or other sterols. -
Sequence similarities
Contains 1 START domain. - Information by UniProt
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Database links
- Entrez Gene: 134429 Human
- Omim: 607049 Human
- SwissProt: Q96DR4 Human
- Unigene: 93842 Human
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Alternative names
- 4632419C16Rik antibody
- 9030213J02Rik antibody
- AA517649 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence analysis of SH-SY5Y (Human neuroblastoma from bone marrow cells) cells labeling STARD4 with ab200344 at a 1/100 dilution. ab150077 AlexaFluor®488 Goat anti-Rabbit secondary, 1/500 dilution. Counterstained using ab7291 anti-Tubulin (mouse mAb), 1/1000 dilution and ab150120 AlexaFluor®594 Goat anti-Mouse secondary, 1/500 dilution. Cells were fixed in 100% methanol and permeabilized using 0.1% Triton X-100. Nuclear stain DAPI.
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All lanes : Anti-STARD4 antibody [EPR17847] (ab200344) at 1/1000 dilution
Lane 1 : SH-SY5Y (Human neuroblastoma from bone marrow cells) whole cell lysate
Lane 2 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
Lane 3 : 293 (Human epithelial cells from embryonic kidney) whole cell lysate
Lane 4 : U-937 (Human histiocytic lymphoma cells) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 24 kDa -
All lanes : Anti-STARD4 antibody [EPR17847] (ab200344) at 1/1000 dilution
Lane 1 : Human fetal heart lysate
Lane 2 : Human fetal kidney lysate
Lane 3 : Human fetal liver lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 24 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab200344 has not yet been referenced specifically in any publications.