Overview

Applications

Our Abpromise guarantee covers the use of ab82636 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 24 kDa.

This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

Target

  • RelevanceSTARD5, or START domain containing 5, belongs to a family containing a 200- to 210-amino acid STAR-related lipid transfer (START) domain. STARD5 may be involved in the intracellular transport of sterols or other lipids and may bind cholesterol or other sterols.
  • Cellular localizationCytoplasmic
  • Database links
  • Alternative names
    • MGC10327 antibody
    • StAR related lipid transfer protein 5 antibody
    • StAR-related lipid transfer (START) domain containing 5 antibody
    • StARD5 antibody
    • START domain containing 5 antibody
    • START domain containing protein 5 antibody
    see all

Anti-STARD5 antibody images

  • Anti-STARD5 antibody (ab82636) at 1/500 dilution + Human macrophage whole cell lysate at 40 µg

    Secondary
    HRP Goat anti-mouse IgG polyclonal at 1/2000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 24 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa (possible non-specific binding),60 kDa (possible non-specific binding),90 kDa (possible non-specific binding).

    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-STARD5 antibody (ab82636)

ab82636 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (macrophages)
Loading amount 40 µg
Specification macrophages
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted May 01 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"