Synthetic phosphopeptide derived from human STAT1 around the phosphorylation site of Tyrosine 701.
Our Abpromise guarantee covers the use of ab30645 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC | Use at an assay dependent concentration. | |
ELISA | 1/10000. | |
IP | Use at an assay dependent concentration. | |
IHC-P | Use at an assay dependent concentration. | |
WB | 1/500 - 1/1000. Detects a band of approximately 87 kDa (predicted molecular weight: 87 kDa). | |
ICC/IF | Use a concentration of 1 - 5 µg/ml. |
Paraffin-embedded human breast carcinoma tissue stained for STAT1 (phospho Y701) using ab30645 at 1/590 dilution in immunohistochemical analysis. Tissue was incubated in the absence (left) or presence (right) of immunizing phospho-peptide.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"