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Synthetic peptide corresponding to Human STAT1 (phospho Y701) conjugated to Keyhole Limpet Haemocyanin (KLH).
Our Abpromise guarantee covers the use of ab29045 in the following tested applications.
|ICC||Use at an assay dependent concentration.|
|WB||1/1000. Predicted molecular weight: 87 kDa.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 21677781|
ab29045 staining STAT1 (phospho Y701) in human SKOV-3 and MDA-MB-468 cells by Immunocytochemistry/ Immunofluorescence. The cells were EDTA/trypsin treated then fixed in 10% formaldehyde and blocked using 2% BSA for 1 hour. Samples were then incubated with primary antibody at 1/100 for 18 hours at 4°C. The secondary antibody used was an anti-mouse IgG conjugated to HRP used at a 1/1000 dilution.
Western blot analysis of human A431 cells untreated (lanes 1, 3, 5, 7, & 9) or treated with EGF (100 nM) for 60 min (lanes 2, 4, 6, 8, & 10).