Synthetic peptide corresponding to Human STAT1 (phospho Y701) conjugated to Keyhole Limpet Haemocyanin (KLH).
Our Abpromise guarantee covers the use of ab29045 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent concentration.|
|WB||1/1000. Predicted molecular weight: 87 kDa.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 21677781|
ab29045 staining STAT1 (phospho Y701) in human SKOV-3 and MDA-MB-468 cells by Immunocytochemistry/ Immunofluorescence. The cells were EDTA/trypsin treated then fixed in 10% formaldehyde and blocked using 2% BSA for 1 hour. Samples were then incubated with primary antibody at 1/100 for 18 hours at 4°C. The secondary antibody used was an anti-mouse IgG conjugated to HRP used at a 1/1000 dilution.
Western blot analysis of human A431 cells untreated (lanes 1, 3, 5, 7, & 9) or treated with EGF (100 nM) for 60 min (lanes 2, 4, 6, 8, & 10).