Anti-STAT1 (phospho Y701) antibody [M135] (ab29045)

Overview

  • Product nameAnti-STAT1 (phospho Y701) antibody [M135]
    See all STAT1 primary antibodies
  • Description
    Mouse monoclonal [M135] to STAT1 (phospho Y701)
  • SpecificityThe antibody detects 84 and 91 kDa stat1 variants on SDS-PAGE immunoblots of human A431 treated with EGF, as well as Jurkat and A431 cells treated with pervanadate. The antibody does not detect these variants in control cells.
  • Tested applicationsSuitable for: ICC, WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to Human STAT1 (phospho Y701) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • Positive control
    • human SKOV-3 and MDA-MB-468 cells, ab7909, Jurkat cells

Properties

Applications

Our Abpromise guarantee covers the use of ab29045 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
WB 1/1000. Predicted molecular weight: 87 kDa.
ELISA 1/2000.
ICC/IF Use at an assay dependent concentration. PubMed: 21677781

Target

  • FunctionSignal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state.
  • Involvement in diseaseNote=STAT1 deficiency results in impaired immune response leading to severe mycobacterial and viral diseases. In the case of complete deficiency, patients can die of viral disease.
    Defects in STAT1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD) [MIM:209950]; also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance.
  • Sequence similaritiesBelongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • Post-translational
    modifications
    Phosphorylated on tyrosine and serine residues in response to IFN-alpha, IFN-gamma, PDGF and EGF. Phosphorylation on Tyr-701 (lacking in beta form) by JAK promotes dimerization and subsequent translocation to the nucleus. Phosphorylation on Ser-727 by several kinases including MAPK14, ERK1/2 and CAMKII on IFN-gamma stimulation, regulates STAT1 transcriptional activity. Phosphorylation on Ser-727 promotes sumoylation though increasing interaction with PIAS. Phosphorylation on Ser-727 by PKCdelta induces apoptosis in response to DNA-damaging agents.
    Sumoylated by SUMO1, SUMO2 and SUMO3. Sumoylation is enhanced by IFN-gamma-induced phosphorylation on Ser-727, and by interaction with PIAS proteins. Enhances the transactivation activity.
    ISGylated.
  • Cellular localizationCytoplasm. Nucleus. Translocated into the nucleus in response to IFN-gamma-induced tyrosine phosphorylation and dimerization.
  • Information by UniProt
  • Database links
  • Alternative names
    • Signal transducer and activator of transcription 1 91kD antibody
    • CANDF7 antibody
    • DKFZp686B04100 antibody
    • IMD31A antibody
    • IMD31B antibody
    • IMD31C antibody
    • ISGF 3 antibody
    • ISGF-3 antibody
    • OTTHUMP00000163552 antibody
    • OTTHUMP00000165046 antibody
    • OTTHUMP00000165047 antibody
    • OTTHUMP00000205845 antibody
    • Signal transducer and activator of transcription 1 91kDa antibody
    • Signal transducer and activator of transcription 1 antibody
    • Signal transducer and activator of transcription 1, 91kD antibody
    • Signal transducer and activator of transcription 1-alpha/beta antibody
    • STAT 1 antibody
    • Stat1 antibody
    • STAT1_HUMAN antibody
    • STAT91 antibody
    • Transcription factor ISGF 3 components p91 p84 antibody
    • Transcription factor ISGF-3 components p91/p84 antibody
    see all

Anti-STAT1 (phospho Y701) antibody [M135] images

  • ab29045 staining STAT1 (phospho Y701) in human SKOV-3 and MDA-MB-468 cells by Immunocytochemistry/ Immunofluorescence. The cells were EDTA/trypsin treated then fixed in 10% formaldehyde and blocked using 2% BSA for 1 hour. Samples were then incubated with primary antibody at 1/100 for 18 hours at 4°C. The secondary antibody used was an anti-mouse IgG conjugated to HRP used at a 1/1000 dilution.

  • Lanes 1, 2 & 4 : Anti-STAT1 antibody (ab2415)
    Lane 3 : Anti-STAT1 (phospho Y701) antibody [M135] (ab29045)
    Lanes 5 - 6 : Anti-STAT3 antibody (ab5073)
    Lanes 7 - 8 : Anti-STAT5 antibody (ab68465)
    Lanes 9 - 10 : anti-Stat5 (Tyr-694)

    Lane 1 : A431 whole cell lysate (ab7909)
    Lane 2 : A431 whole cell lysate (ab7909)
    Lane 3 : A431 whole cell lysate (ab7909)
    Lane 4 : A431 whole cell lysate (ab7909)
    Lane 5 : A431 whole cell lysate (ab7909)
    Lane 6 : A431 whole cell lysate (ab7909)
    Lane 7 : A431 whole cell lysate (ab7909)
    Lane 8 : A431 whole cell lysate (ab7909)
    Lane 9 : A431 whole cell lysate (ab7909)
    Lane 10 : A431 whole cell lysate (ab7909)


    Predicted band size : 87 kDa

    Western blot analysis of human A431 cells untreated (lanes 1, 3, 5, 7, & 9) or treated with EGF (100 nM) for 60 min (lanes 2, 4, 6, 8, & 10).

  • Lanes 1 - 2 : Anti-Stat1
    Lanes 3 - 4 : Anti-STAT1 (phospho Y701) antibody [M135] (ab29045) at 1/1000 dilution

    Lane 1 : Jurkat cells
    Lane 2 : Jurkat cells treated with 1mM pervanadate
    Lane 3 : Jurkat cells
    Lane 4 : Jurkat cells treated with 1mM pervanadate


    Predicted band size : 87 kDa
    Observed band size : 84,91 kDa (why is the actual band size different from the predicted?)

References for Anti-STAT1 (phospho Y701) antibody [M135] (ab29045)

This product has been referenced in:
  • Rivera LE  et al. Cystatin B and HIV regulate the STAT-1 signaling circuit in HIV-infected and INF-ß-treated human macrophages. J Neurovirol 22:666-673 (2016). Read more (PubMed: 27137788) »
  • Stone AE  et al. Hepatitis C virus core protein inhibits interferon production by a human plasmacytoid dendritic cell line and dysregulates interferon regulatory factor-7 and signal transducer and activator of transcription (STAT) 1 protein expression. PLoS One 9:e95627 (2014). WB . Read more (PubMed: 24788809) »

See all 7 Publications for this product

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Hep3B cells)
Loading amount 10 µg
Specification Hep3B cells
Treatment 10 ng/ml IFNg
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 03 2012

ab29045 positive control would be either human A431 cells treated with EGF (100 nM for 60 min) as well as Jurkat and A431 cells treated with pervanadate (1 mM for 30 min).

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Human B-cell lymphomas)
Loading amount 20 µg
Specification Human B-cell lymphomas
Gel Running Conditions Reduced Denaturing (8% Acrylamide gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Prof. Paul Townsend

Verified customer

Submitted Mar 31 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Human Cultured Cells (Ovarian, Breast)
Specification Ovarian, Breast
Fixative Formaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2%
Username

Shunyou Wang

Verified customer

Submitted Mar 31 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Renal and Breast)
Loading amount 8 µg
Specification Renal and Breast
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Mar 04 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"