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A synthetic peptide corresponding to residues near N-terminus of human Stat-2.
This product is a recombinant rabbit monoclonal antibody.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Our Abpromise guarantee covers the use of ab32367 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/10000. Detects a band of approximately 98 kDa (predicted molecular weight: 98 kDa).|
|ICC||1/50 - 1/100.|
|IHC-P||1/100 - 1/250.|
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: STAT2 knockout HAP1 whole cell lysate (20 µg)
Lane 3: K562 whole cell lysate (20 µg)
Lane 4: THP1 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab32367 observed at 97 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab32367 was shown to recognize STAT2 in wild-type HAP1 cells as signal was lost at the expected MW in STAT2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and STAT2 knockout samples were subjected to SDS-PAGE. Ab32367 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
ab32367 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"