Anti-STAT2 (phospho Y690) antibody (ab53132)
Key features and details
- Rabbit polyclonal to STAT2 (phospho Y690)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-STAT2 (phospho Y690) antibody
See all STAT2 primary antibodies -
Description
Rabbit polyclonal to STAT2 (phospho Y690) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human STAT2 aa 650-750 (phospho Y690). Around the phosphorylation site of tyrosine 690 (R-K-YP-L-K).
Database link: P52630 -
Positive control
- IHC-P: Rat kidney, Human colon. WB: HeLa cells treated with IFN.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab53132 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab53132 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/300 - 1/1000. Detects a band of approximately 98 kDa (predicted molecular weight: 98 kDa).
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IHC-P | (1) |
Use at an assay dependent concentration.
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Notes |
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WB
1/300 - 1/1000. Detects a band of approximately 98 kDa (predicted molecular weight: 98 kDa). |
IHC-P
Use at an assay dependent concentration. |
Target
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Function
Signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. -
Sequence similarities
Belongs to the transcription factor STAT family.
Contains 1 SH2 domain. -
Post-translational
modificationsTyrosine phosphorylated in response to IFN-alpha. -
Cellular localization
Cytoplasm. Nucleus. Translocated into the nucleus upon activation by IFN-alpha/beta. - Information by UniProt
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Database links
- Entrez Gene: 6773 Human
- Omim: 600556 Human
- SwissProt: P52630 Human
- Unigene: 530595 Human
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Alternative names
- Homo sapiens interferon alpha induced transcriptional activator antibody
- interferon alpha induced transcriptional activator antibody
- ISGF 3 antibody
see all
Images
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All lanes : Anti-STAT2 (phospho Y690) antibody (ab53132) at 1/300 dilution
Lane 1 : HeLa cell extract treated with IFN (2500U/ml, 30mins)
Lane 2 : HeLa cell extract treated with IFN (2500U/ml, 30mins) with immunising peptide
Predicted band size: 98 kDa
Observed band size: 98 kDa -
ab53132, at 1/50 dilution, staining STAT2 in paraffin embedded human lung carcinoma tissue by Immunohistochemistry in the absence (left image) or presence (right image) of the immunising peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (24)
ab53132 has been referenced in 24 publications.
- Xu S et al. Leukemia inhibitory factor is a therapeutic target for renal interstitial fibrosis. EBioMedicine 86:104312 (2022). PubMed: 36335669
- Liang Y et al. Curcumin inhibits the viability, migration and invasion of papillary thyroid cancer cells by regulating the miR-301a-3p/STAT3 axis. Exp Ther Med 22:875 (2021). PubMed: 34194553
- Wong SW et al. Global deletion of Optineurin results in altered type I IFN signaling and abnormal bone remodeling in a model of Paget's disease. Cell Death Differ 27:71-84 (2020). PubMed: 31076632
- LaClair KD et al. Congenic expression of poly-GA but not poly-PR in mice triggers selective neuron loss and interferon responses found in C9orf72 ALS. Acta Neuropathol 140:121-142 (2020). PubMed: 32562018
- Jena KK et al. Autoimmunity gene IRGM suppresses cGAS-STING and RIG-I-MAVS signaling to control interferon response. EMBO Rep 21:e50051 (2020). PubMed: 32715615