Our Abpromise guarantee covers the use of ab30647 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 20950279|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.|
|WB||1/500 - 1/1000. Detects a band of approximately 88 kDa (predicted molecular weight: 88 kDa).Can be blocked with Recombinant Human STAT3 protein (ab43618).|
|IP||Use at an assay dependent concentration.|
PFA-fixed frozen rat liver tissue stained for STAT3 (phospho S727) using ab30647 at 1/1000 dilution in immunohistochemical analysis, followed by Alexa Fluor® 488 donkey anti-rabbit.