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STAT3 (pY705) + total STAT3 ELISA Kit (ab126459) is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cell lysates. By determining phosphorylated STAT3 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blot analysis.
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of phospho-STAT3 (Tyr705) and total STAT3 in human, mouse and rat cell lysates (help normalize the results of phospho-STAT3 from different cell lysate being compared). A pan STAT3 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and STAT3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and anti-phospho-STAT3 (Tyr705) is used to detect phosphorylated STAT3 or biotinylated anti-total-STAT3 is used to detect total STAT3. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG or HRP-streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of STAT3 (Tyr705) or total STAT3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get results in 90 minutes with STAT3 (pY705 + Total) ELISA Kit (ab176666) from our SimpleStep ELISA® range.
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|2x Cell lysate buffer||1 x 5ml|
|5X Assay Diluent||1 x 15ml|
|80x HRP-Streptavidin Concentrate||1 x 200µl|
|Detection Antibody STAT3||1 vial|
|Detection Antibody STAT3 (Tyr705)||1 vial|
|HRP-conjugated anti-rabbit IgG||1 x 25µl|
|Positive Control: lyophilized powder from A431 cell lysate||1 vial|
|STAT3 Microplate (12 strips x 8 wells) coated with anti-pan STAT3 antibody||1 unit|
|Stop Solution||1 x 8ml|
|TMB Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab126459 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|