Overview

  • Product nameAnti-STAT4 antibody
    See all STAT4 primary antibodies
  • Description
    Rabbit polyclonal to STAT4
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic non phosphopeptide derived from human STAT4 around the phosphorylation site of Tyrosine 693.

  • Positive control
    • IHC-P: Breast carcinoma tissue. WB: HeLa cell extract.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab31376 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 86 kDa. (When this protein gets phosphorylated, it runs as lower MW on SDS-PAGE).
ELISA 1/4000.
IHC-P Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

  • FunctionCarries out a dual function: signal transduction and activation of transcription. Involved in IL12 signaling.
  • Involvement in diseaseSystemic lupus erythematosus 11
    Rheumatoid arthritis
  • Sequence similaritiesBelongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • Post-translational
    modifications
    Tyrosine phosphorylated. Serine phosphorylation is also required for maximal transcriptional activity.
  • Cellular localizationCytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
  • Information by UniProt
  • Database links
  • Alternative names
    • Signal transducer and activator of transcription 4 antibody
    • SLEB11 antibody
    • STAT4 antibody
    • STAT4_HUMAN antibody

Anti-STAT4 antibody images

  • Ab31376, at a dilution of 1/50, staining STAT4 in paraffin embedded breast carcinoma tissue by Immunohistochemistry.
    Left image: Using Ab31376.
    Right image: The same antibody preincubated with synthesized peptide.
  • Lane 1 : Anti-STAT4 antibody (ab31376) at 1/500 dilution
    Lane 2 : Anti-STAT4 antibody (ab31376) at 1/500 dilution (preincubated with synthesized peptide)

    Lane 1 : HeLa cell extract (5-30ug).
    Lane 2 : HeLa cell extract (5-30ug).


    Predicted band size : 86 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)

References for Anti-STAT4 antibody (ab31376)

ab31376 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"