• Product nameAnti-STAT4 antibody
    See all STAT4 primary antibodies
  • Description
    Mouse monoclonal to STAT4
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-749 of Human STAT4



Our Abpromise guarantee covers the use of ab57822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 86 kDa.
ICC/IF Use a concentration of 1 - 5 µg/ml.
IHC-P Use a concentration of 2 µg/ml.
Flow Cyt Use 2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


  • FunctionCarries out a dual function: signal transduction and activation of transcription. Involved in IL12 signaling.
  • Involvement in diseaseSystemic lupus erythematosus 11
    Rheumatoid arthritis
  • Sequence similaritiesBelongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • Post-translational
    Tyrosine phosphorylated. Serine phosphorylation is also required for maximal transcriptional activity.
  • Cellular localizationCytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
  • Information by UniProt
  • Database links
  • Alternative names
    • Signal transducer and activator of transcription 4 antibody
    • SLEB11 antibody
    • STAT4 antibody
    • STAT4_HUMAN antibody

Anti-STAT4 antibody images

  • STAT4 antibody (ab57822) used in immunofluorescence at 1-5ug/ml on HeLa cells.
  • STAT4 antibody (ab57822) used in immunohistochemistry at 2ug/ml on formalin fixed and paraffin embedded human ovary, clear cell carcinoma tissue.

  • Predicted band size : 86 kDa
    STAT4 antibody (ab57822) at 1ug/lane + A-549 cell lysate at 25ug/lane.
  • Overlay histogram showing HeLa cells stained with ab57822 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57822, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-STAT4 antibody (ab57822)

ab57822 has not yet been referenced specifically in any publications.

Product Wall

Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for...

Read More