Recombinant
RabMAb

Anti-STAT4 antibody [EP1900Y] (ab68156)

Overview

  • Product name
    Anti-STAT4 antibody [EP1900Y]
    See all STAT4 primary antibodies
  • Description
    Rabbit monoclonal [EP1900Y] to STAT4
  • Tested applications
    Suitable for: WB, IP, IHC-P, ICC, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human STAT4 aa 700 to the C-terminus. The exact sequence is proprietary.
    Database link: Q14765

  • Positive control
    • WB: Daudi, K562 and Hela cell lysates. IHC-P: Human tonsil tissue sections.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab68156 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000. Detects a band of approximately 30, 86 kDa (predicted molecular weight: 86 kDa).
IP 1/20.
IHC-P 1/100 - 1/250.
ICC 1/100 - 1/250.
Flow Cyt 1/80.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Carries out a dual function: signal transduction and activation of transcription. Involved in IL12 signaling.
  • Involvement in disease
    Systemic lupus erythematosus 11
    Rheumatoid arthritis
  • Sequence similarities
    Belongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • Post-translational
    modifications
    Tyrosine phosphorylated. Serine phosphorylation is also required for maximal transcriptional activity.
  • Cellular localization
    Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
  • Information by UniProt
  • Database links
  • Alternative names
    • Signal transducer and activator of transcription 4 antibody
    • SLEB11 antibody
    • STAT4 antibody
    • STAT4_HUMAN antibody

Images

  • All lanes : Anti-STAT4 antibody [EP1900Y] (ab68156) at 1/1000 dilution

    Lane 1 : Daudi cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : Hela cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 86 kDa
    Observed band size : 86 kDa
    Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.
  • ab68156, at 1/100 dilution, staining STAT4 in paraffin embedded human tonsil tissue sections by Immunohistochemistry

References

ab68156 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (human Vgamma9 Vdelta2 T cells)
Total protein in input
3e+006 cells
Immuno-precipitation step
Other - Dynabeads (Protein G)
Specification
human Vgamma9 Vdelta2 T cells
Username

Abcam user community

Verified customer

Submitted Nov 07 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (Purified human Vgamma9 Vdelta2 T-cells)
Permeabilization
Yes - Foxp3 Staining Buffer Set
Gating Strategy
CD3 and V delta 2 positive population
Specification
Purified human Vgamma9 Vdelta2 T-cells
Fixation
Foxp3 Staining Buffer Set
Username

Abcam user community

Verified customer

Submitted Sep 26 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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