Overview

  • Product name
  • Description
    Rabbit polyclonal to STAT5
  • Specificity
    This antibody does not cross react with any other STATs or related proteins
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep, Cow
  • Immunogen

    Synthetic peptide:

    ATIISEQQAKSLLKNENTR

    , corresponding to amino acids 371-389 of Human STAT5.

  • Positive control
    • CTLL cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab16276 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 90 kDa. Detects bands of approximately 92 kDa and 85 kDa representing STAT5a and STAT5b respectively from CTLL cell lysate.

Target

Images

  • Anti-STAT5 antibody (ab16276) + SK-BR-3 Human adenocarcinoma cell line

    Secondary
    HRP conjugated goat anti-rabbit

    Predicted band size : 90 kDa
  • ICC/IF image of ab71498 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16276, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Elliott S  et al. Lack of expression and function of erythropoietin receptors in the kidney. Nephrol Dial Transplant : (2011). WB ; Human . Read more (PubMed: 22167585) »
  • Venken K  et al. Growth without growth hormone receptor: estradiol is a major growth hormone-independent regulator of hepatic IGF-I synthesis. J Bone Miner Res 20:2138-49 (2005). Read more (PubMed: 16294267) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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