Overview

  • Product name
  • Description
    Rabbit polyclonal to STAT5
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Sheep, Cow
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human STAT5.

    (Peptide available as ab99165.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: MCF7; Jurkat.

Properties

Applications

Our Abpromise guarantee covers the use of ab88246 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).

Target

  • Function
    Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.
  • Sequence similarities
    Belongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • Post-translational
    modifications
    Tyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity.
  • Cellular localization
    Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
  • Information by UniProt
  • Database links
  • Alternative names
    • MGF antibody
    • Signal transducer and activator of transcription 5A antibody
    • Signal Transducer and Activator of Transcription 5B antibody
    • STA5A_HUMAN antibody
    • STAT 5A antibody
    • STAT 5B antibody
    • STAT5 antibody
    • STAT5A antibody
    • STAT5B antibody
    • Transcription factor STAT5A antibody
    • Transcription factor STAT5B antibody
    see all

Images

  • All lanes : Anti-STAT5 antibody (ab88246) at 1 µg/ml

    Lane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 90 kDa
    Observed band size : 90 kDa
    Additional bands at : 140 kDa,68 kDa. We are unsure as to the identity of these extra bands.

References

ab88246 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab88246.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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