Overview

  • Product nameAnti-STAT5 (phospho Y694) antibody
    See all STAT5 primary antibodies
  • Description
    Rabbit polyclonal to STAT5 (phospho Y694)
  • SpecificityThis antibody recognizes STAT5 (pY694) with phosphorylated sites of Tyr694. It does not cross react with non phosphospecific peptide.
  • Tested applicationsSuitable for: ELISA, IHC-P, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Dog
  • Immunogen

    A synthetic peptide surrounding the epitope -DGYVK- with phosphorylation sites at Tyr694 of STAT5 protein from Human, Mouse and Rat origins.

  • Positive control
    • GH stimulated Mouse liver lysate and Human breast carcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab97734 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.01 - 0.1 µg/ml.
IHC-P 1/100. Staining of formalin fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.
IP Use a concentration of 2 - 5 µg/ml.
WB Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 90 kDa.

Target

Anti-STAT5 (phospho Y694) antibody images

  • Human breast carcinoma tissue stained with ab97734 at 1/100 for 10 minutes at room temperature.
  • All lanes : Anti-STAT5 (phospho Y694) antibody (ab97734) at 1/500 dilution

    Lane 1 : GH stimulated Mouse liver lysate
    Lane 2 : Negtive control


    Predicted band size : 90 kDa

References for Anti-STAT5 (phospho Y694) antibody (ab97734)

This product has been referenced in:
  • Tsuruyama T  et al. STAT5A Modulates Chemokine Receptor CCR6 Expression and Enhances Pre-B Cell Growth in a CCL20-Dependent Manner. J Cell Biochem 117:2630-42 (2016). Read more (PubMed: 27018255) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"