Overview

  • Product name
  • Description
    Rabbit polyclonal to STAT5a
  • Specificity
    ab31377 detects endogenous levels of STAT5A protein around Tyrosine 694.
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthesized non-phosphopeptide derived from human STAT5A around the phosphorylation site of Tyrosine 694.

  • Positive control
    • HeLa cell lysate, Breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab31377 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
WB 1/500 - 1/1000. Predicted molecular weight: 91 kDa.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.
  • Sequence similarities
    Belongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • Post-translational
    modifications
    Tyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity.
  • Cellular localization
    Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
  • Information by UniProt
  • Database links
  • Alternative names
    • Mammary gland factor antibody
    • MGF antibody
    • Signal transducer and activator of transcription 5A antibody
    • STA5A_HUMAN antibody
    • STAT 5 antibody
    • STAT 5A antibody
    • STAT5 antibody
    • STAT5A antibody
    see all

Images

  • Lane 1 : Anti-STAT5a antibody (ab31377) at 1/500 dilution
    Lane 2 : Anti-STAT5a antibody (ab31377) at 1/500 dilution (preincubated with synthesized peptide)

    Lane 1 : HeLa cell extract.
    Lane 2 : HeLa cell extract.


    Predicted band size : 91 kDa
    Observed band size : 89 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 83 kDa. We are unsure as to the identity of these extra bands.

References

ab31377 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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