Overview

  • Product name
  • Description
    Rabbit polyclonal to STAT5a
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Three synthetic peptides (Human) conjugated to KLH

  • Positive control
    • An induced culture of E. coli

Properties

Applications

Our Abpromise guarantee covers the use of ab86030 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: 1/1000. Detects a band of approximately 105 kDa (predicted molecular weight: 91 kDa). Tag is 14kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.
    • Sequence similarities
      Belongs to the transcription factor STAT family.
      Contains 1 SH2 domain.
    • Post-translational
      modifications
      Tyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity.
    • Cellular localization
      Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
    • Information by UniProt
    • Database links
    • Alternative names
      • Mammary gland factor antibody
      • MGF antibody
      • Signal transducer and activator of transcription 5A antibody
      • STA5A_HUMAN antibody
      • STAT 5 antibody
      • STAT 5A antibody
      • STAT5 antibody
      • STAT5A antibody
      see all

    Images

    • All lanes : Anti-STAT5a antibody (ab86030) at 1/1000 dilution

      Lane 1 : An un-induced culture of E. coli
      Lane 2 : An induced culture of E. coli

      Lysates/proteins at 1/5000 dilution per lane.

      Secondary
      Anti-Rabbit IgG HRP at 1/1000 dilution

      Predicted band size : 91 kDa
      Observed band size : 49,75,105 kDa (why is the actual band size different from the predicted?)


      Exposure time : 60 minutes

    References

    ab86030 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab86030.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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